Maspin和mmp-2在非小细胞肺癌中的表达及其临床意义

Expressions of maspin and mmp-2 in non-small cell lung cancer and their clinical significance

目的:研究肿瘤抑制基因蛋白maspin(Mammary-serpin)和基质金属蛋白酶-2(Matrix metalloproteinase-2,mmp-2)在非小细胞肺癌(Non-small cell lungcancer,NSCLC)中的表达,探讨maspin和mmp-2的相关性及其与临床病理特征的关系。方法:选择手术切除的65例NSCLC患者的标本,肺部良性病变组织及正常肺组织标本各8例,采用免疫组化SP法检测NSCLC组织、肺部良性病变及正常肺组织中maspin和mmp-2的表达情况。结果:maspin蛋白在正常肺组织和肺部良性病变组织中高表达,阳性率分别为100%(8/8)和87.5%(7/8),在NSCLC中其阳性率显著降低,为15.4%(10/65)。mmp-2在正常肺组织和肺部良性病变组织中不表达或低表达,阳性率分别为12.5%(1/8)和37.5%(3/8),而其在NSCLC中则显著高表达,阳性率为73.3%。maspin蛋白在NSCLC的表达与淋巴结转移相关,mmp-2在NSCLC的表达与淋巴结转移和TNM分期相关,两者与性别、年龄、肿瘤大小、组织学类型和组织分化程度不相关。结论:maspin蛋白在NSCLC中表达下调,mmp-2在NSCLC中过度表达,并且maspin蛋白与mmp-2的表达成负相关(rs=-0.54,P=0.018)。它们在肺癌浸润转移中起重要作用,可作为反映肺癌病理生物学行为的有效指标。

Objective：To investigate the expressions of maspin and the matrix metalloproteinase-2 and correlation between maspin and mmp-2 protein in NSCLC and their clinical significance.Methods： Immunohistochemical SP method was used to detect the expressions of maspin and mmp-2 in 65 NSCLC tssues,8 tissues of benign disease of lung,and 8 normal lung tissues.Results：Maspin protein in normal lung tissues and benign lesions in the lungs were of high expression：the positive rates were 100%（8/8） and 87.5%（7/8）respectively.The positive rate of NSCLC showed a significant reduction which was 15.4%（10/65）.mmp-2 in normal lung tissues and benign lesions of the lungs did not express or showed low-expression： the positive rates were 12.5%（1/8） and 37.5%（3/8）respectively,and its expression in NSCLC was significantly higher,which is 73.3%.Maspin protein expression in NSCLC had a correlation with lymph node metastasis,and mmp-2 expression in NSCLC had a correlation with lymph node metastasis and TNM staging.Both maspin protein and mmp-2 did not show correlation between the two genders,age,tumor size,or histological type.Conclusion：The expression of maspin protein in NSCLC is down-regulated and mmp-2 shows an over-expression in NSCLC.The expression of Maspin protein and mmp-2 has a negative correlation（rs=-0.54,P=0.018）.They play an important role in invasion and metastasis in lung cancer.They can be an effective indicator of biological behavior which can reflect lung cancer pathology.