利胆止痛胶囊对小鼠抗抑菌炎、镇痛和利胆作用研究

Anti-inflammatory,Analgesic and Choleretic Effect of Lidan Zhitong Capsulen Mice

目的观察云南永孜堂制药有限公司生产的利胆止痛胶囊的利胆、抑菌和抗炎镇痛作用.方法采用摘除小鼠胆囊直接称重的方法观测利胆止痛胶囊对正常小鼠胆汁量的影响.采用试管两倍稀释法观测利胆止痛胶囊在体外对甲型溶血性链球菌、乙型溶血性链球菌、金黄色葡萄球菌及痢疾杆菌、大肠杆菌和沙门菌生长的影响.采用小鼠耳廓肿胀、大鼠足跖肿胀模型,观测利胆止痛胶囊抗炎消肿作用.运用小鼠热板法及醋酸扭体方法评价利胆止痛胶囊的镇痛作用.结果 0.72、1.44 g/kg的利胆止痛胶囊明显减轻正常小鼠的胆囊重量,低剂量的利胆止痛胶囊对小鼠胆囊重量无明显影响.利胆止痛胶囊对金黄色葡萄球菌、甲型溶血性链球菌、乙型溶血性链球菌、痢疾杆菌和大肠杆菌的MIC分别为25、50、50、25和12.5 mg/mL,对沙门菌无明显抑菌作用.0.72、1.44 g/kg利胆止痛胶囊明显减轻小鼠耳廓肿胀度.对大鼠足跖肿胀模型,高剂量(0.72 g/kg)的利胆止痛胶囊于给药后1 h起效,作用至少可持续4 h;中、低剂量无明显影响.0.72和1.44 g/kg的利胆止痛胶囊明显减少小鼠扭体次数,热板法结果表明于给药后60～120 min均显著延长小鼠置入圆筒至开始舔后足时间.结论利胆止痛胶囊具有抑菌、抗炎、消肿、镇痛和利胆作用.

Objective To investigate the anti-inflammatory,analgesic and choleretic effect of Lidan zhitong capsule.Methods The mouse gallbladder was removed after medication and weighed directly to measure the effect of Lidan zhitong capsule on bile secretion and excretion.The test tube dilution method was used to observe the bacteriostatic effect on staphylococcus aureus,alpha hemolytic streptococcus,beta-hemolytic streptococci,shigella dysenteriae,escherichia coli and salmonella.The anti-inflammation and detumescence of Lidan zhitong capsule were evaluated by using the xylene-caused mouse ear swelling and carrageenin-challenged paw edema,respectively.The analgesic effect was evaluated by the hot plate and the acetic acid twist in mice.Results Lidan zhitong capsule at 0.72 and1.44 g/kg significantly decreased the weight of mouse gallbladders,while 0.36 g/kg of Lidan zhitong capsule had no effect.The minimal inhibitory concentrations,MIC）of Lidan zhitong capsule on staphylococcus aureus,alpha hemolytic streptococcus,beta-hemolytic streptococci,shigella dysenteriae and escherichia coli were 25 mg/mL,50 mg/mL,50 mg/mL,25 mg/mLand 12.5 mg/mL,respectively.0.72 and1.44 g/kg of Lidan zhitong capsule lightened the dimethylxylene-caused mouse ear swelling and carrageenin-challenged paw edema in rats,in a dose-dependent manner.Lidan zhitong capsule at 0.72 and 1.44 g/kg obviously decreased twist times of the mice.After the mice had been medicated 60～120 min,Lidan zhitong capsule,0.72 and 1.44 g/kg）significantly increased the time that the mice began to lick the hindleg after the mice inserted in the cylinder.Conclusion Lidan zhitong capsule has obvious choleretic effect,bacteriostasis on common pathogenic bacteria in digestive tract,and significant anti-inflammatory,detumescent effect as well as analgesic effect.