高脂血症肾损害大鼠体内辅脂酶的变化

Changes of colipase in lipid-renal damage rats

目的观察高脂血症大鼠血脂、尿蛋白、血肌酐,以及血清和肾皮质辅脂酶浓度及辛伐他汀治疗后的影响,探讨辅脂酶在高脂血症大鼠肾损害中可能的作用机制。方法将30只SD大鼠随机分为正常对照组、高脂血症模型组和辛伐他汀(10mg·kg-1d-1)治疗组,每组10只,12周后检测三组大鼠的血脂、血肌酐、24h尿蛋白水平以及血清和肾皮质辅脂酶浓度。结果高脂血症组尿蛋白明显高于正常对照组(P<0.01),肾组织辅脂酶水平低于正常对照组(P<0.05)。辛伐他汀治疗组总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-C)、24h尿蛋白定量显著低于高脂血症组(P<0.01),血清及肾组织colipase含量高于高脂血症组。结论辅脂酶可能参与了高脂血症肾损害的发生,辛伐他汀可能通过调节辅脂酶水平来保护高脂血症肾损害。

Objective To study the mechanism of Simvastatin in protecting lipid-renal damage rats by observing the serum levels of lipid,Cr,urine protein and colipase content in serum and renal cortex.Methods Thirty rats were randomly divided into 3 groups：control group,hyperlipidemic group（HL-untreated） and Simvastatin-treated group（HL-treated,10 mg·kg-1d-1）.The levels of lipid,Cr,urine protein and colipase content in serum and renal cortex were measured.Results Hyperlipidemic rats had higher level of urine protein and lower level of colipase in serum and renal cortex compared with those of control subjects.Significant decrease of level of urine protein and serum lipid and increase of colipase in serum and renal cortex were found in Simvastatin-treated rats.Conclusion Colipase may be involved in lipid-renal damage.Simvastatin can protect renal damage by regulating the level of colipase.