2010年广州某医疗中心儿童夏季细菌性腹泻的病原学调查

Investigation on etiology of bacterial diarrhea of children in summer in Guangzhou in 2010

目的研究广州地区小儿夏季细菌性腹泻的病原菌分布。方法采集2010年5～7月广州市妇女儿童医疗中心珠江新城院区腹泻患儿的大便标本进行常规病原菌的分离培养,通过生化反应和血清凝集试验进行鉴定和分型,并使用金标法对空肠弯曲菌抗原进行检测。结果从110份标本中检出44株病原菌,检出率为40.0%。其中致病性大肠埃希菌17株,2岁以下患儿检出15株;空肠弯曲菌12株,2岁以下患儿检出10株;沙门菌6株;念珠菌纯生长6株;产气荚膜杆菌3株。结论广州地区夏季儿童细菌性腹泻的主要病原菌是致病性大肠埃希菌及空肠弯曲菌,两者的易感人群以2岁以下婴幼儿为主。

Objective To investigate the etiology of bacterial diarrhea of children in summer in Guangzhou in 2010.Methods Children with initial clinical diagnosis of acute bacterial diarrhea during May to July in Guangzhou women and children＇s medical centre of Zhujiang new town hospital were enrolled in 2010.Enteric pathogenic bacteria were isolated,cultured,and identified by biochemistry reaction and serum agglutination test from flesh stool specimens.Campylobacter jejuni antigen was detected by gold colloid methods.Results 44 pathogenic bacteria were detected in 110 stool specimens;the positive rate was 40.0%.17 Enteropathogenic Escherichia coli were isolated,among them 15 strains were isolated from children under 2 year;12 Campylobacter jejuni were detected,10 strains were detected from children under 2 year;6 Salmonella,6 Candida,3 Clostridium perfringens were detected.Conclusion Enteropathogenic Escherichi coli and Campylobacter jejuni were the major pathogens among children with summer bacterial diarrhea in Guangzhou in 2010.children under 2 years were susceptible to these two species of bacteria.