毛细管电泳分离与测定尿液中扑尔敏对映体

Enantioselective Determination and Quantification of Chlorpheniramine in Human Urine by Capillary Electrophoresis

以HP-β-CD作为手性分离添加剂,对标样和实际尿液中的扑尔敏进行了手性分离和测定研究.在实际样品的分离测定时,用液液萃取进行样品预处理,并采用电堆集进样以提高检测灵敏度,对于尿液中扑尔敏对映体的检测限为1.5×10^(-7)g/L.定量线性范围为2.5×10^(-4)～1.5 ×10^(-2)g/L,线性相关系数为0.9965,电泳迁移时间和峰面积的相对标准偏差(RSD%)分别为1.67(n=6)和4.19(n=7).对口服扑尔敏片剂后的排泄尿液中的对映体浓度及组成变化进行了测定.

Chiral separation and determination of basic racemic drug chlopheniramine in urine by capillary electrophoresis was investigated. After oral administration of 4. 0 mg chlopheniramine the enantiomers excretion urine was collected within 24 hours. The extraction sample solution was chiral resoluted using a 50 mmol/L Tris-H3PO4 run buffer (pH 3.0) containing 40 mmol/L hydroxypropyl-B-cyclodextrin. Sample stacking injection was applied to decrease the detection limit. The concentration of chlorpheniramine enantiomers in urine was quantified without adding internal standard. Good linearity, low detection limit and fine precision were obtained.