碱性去活性柱液相色谱测定水溶性维生素

Determination of Water Soluble Vitamins by High Performance Liquid Chromatography on Base Deactivated Column

采用碱性去活性柱(BDS-C_(18))高效液相色谱法同时分离测定了7种水溶性维生素.不需加入离子对试剂和胺类改性剂,峰形尖锐,用梯度洗脱分离完全,效果良好.方法的精密度为相对标准偏差5%以内,回收率为89%～102%,并用于实际样品的测定.

Seven water-soluble vitamins, vitamin B1, R2, B6, B12, nicotinamide, niacin, and folic acid were simultaneously determined by using HPLC on BDS-C18 column instead of C18 column. The detectivewavelengths of ultraviolet detector are 254 nm、 290 nm and 360 nm. Good separation and sharp peaks canbe obtained, and there is no need to add ion pair reagent and triethylamine in mobile phase. The RSD isless than 5% and the recovery is in the range of 89%-102%. The method has been applied to theanalysis of actual samples.