用基质辅助激光解吸电离飞行时间质谱法测定钙调蛋白的纯度与分子量

Determination of Purity and Molecular Weight of Calmodulin by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

采用基质辅助激光解吸电离飞行时间质谱法测定了钙调蛋白的纯度与分子量,并对所得的结果进行了讨论,实验结果表明本方法具有灵敏度高、分析速度快、重复性好、信息直观等特点,是其他传统测定蛋白质分子量的方法无法比拟的.

The purity and molecular weight of calmodulin have been determined by means of matrix-assisted laser desorption/ionization time of flight mass spectrometry, and the results have been discussed, The experimental results demonstrate that this method is high sensitive and rapid as compared with other traditional methods.