以β-环糊精聚合物的超分子包结物为固定化介质的安培型过氧化氢传感器的研究

An Amperometric Hydrogen Peroxide Sensor Based on Supramolecular Inclusion Complex of β CD Polymer as Immobilization Matrix

阐述了一种新的生物传感器的固定化方法．利用β－环糊精聚合物（β－ＣＤＰ）与亚甲基蓝间的超分子作用及β－ＣＤＰ与戊二醛间的缩聚作用，将介体和酶共同固定在电极上，制成的过氧化氢传感器有良好的稳定性和充分的电子流动性，对１．０μｍｏｌ／Ｌ～１．１ｍｍｏｌ／ＬＨ２Ｏ２呈线性响应，检出限为０．

A novel immobilization approach in the fabrication of hydrogen peroxide is described. Methylene blue and enzyme were co immobilized on the electrode by supramolecular function between β CD polymer( β CDP) and cation dyes, as well as condensation polymerization between β CDP and glutaric dialdehye. The fabricated H 2O 2 sensor exhibited excellent stability and efficient shuttled electron between immobilized horseradish peroxidase(HRP) and the electrode, and responded to H 2O 2 in the linear range from 1 0 μmol/L to 1 1 mmol/L with detection limit of 0 5 μmol/L.