PiWil2在甲状腺乳头状癌中的表达及其与侵袭转移的关系侵袭转移的关系

Expression of Piwil2 and its relationship with tumor invasion and metastasis in papillary thyroid carcinoma

目的探讨piwil2在甲状腺乳头状癌（papillary thyroid carcinoma，PTC）中的表达及其与PTC的关系。方法采用免疫组化、原位杂交检测60例PTC患者肿瘤组织及其癌旁组织中Piwil2蛋白和piwil2mRNA的表达情况。结果Piwil2蛋白在肿瘤组织和癌旁组织中的阳性表达率分别为88．3％（53／60）和10．O％（6／60），两者比较差异有统计学意义（x^2=73．654，P〈0．01）。piwil2 mRNA在肿瘤组织和癌旁组织中的阳性表达率分别为85．O％（51／60）和6．7％（4／60），两者比较差异有统计学意义（x^2：74．148，P〈0．01）。Piwil2蛋白及mRNA在PTC中的表达与患者的TNM分期及淋巴转移有关（P值均〈0．05）。Piwil2蛋白及mRNA在PTC中的表达有相关性（x^2=15．098，P〈0．01）。结论Piwil2参与了PTC的发生发展，与PTC的浸润和颈淋巴转移有关。

Objective To study the expressions of Piwil2 protein and mRNA in papillary thyroid carelnoma（PTC） and the relationship between Piwil2 and the invasion and metastasis of PTC. Methods Immunohistoehemistry and in situ hybridization were used to detect the expression of Piwil2 protein and mRNA in 60 cases of PTC with the matched adjacent non-cancerous epithelium （NCE）. Results The positive rates of Piwil2 protein expression in PTC and NCE were 88.3% （53/60） and 10.0% （6/60） respectively, with significant difference （ X^2 = 73. 654, P 〈 0.01 ）. The positive rates of Piwil2 mRNA expression in PTC and NCE were 85.0% （51/60） and 6.7% （4/60） respectively, also with significant difference （X^2 = 74. 148 ,P 〈 0.01 ）. Up-regulated expressions of Piwil2 protein and mRNA were related to the invasion and metastasis of PTC （ P 〈 0.05 ）. Conclusion Piwil2 may play a role in the invasion and metastasis of PTC.