多糖联合对辐照大鼠睾丸组织损伤保护作用

Effect of combined polysaccharides on radiation-induced damage of testis tissue in rats

目的探讨枸杞多糖(LBP)和海带多糖(LJP)联合作用对慢性电离辐射致大鼠睾丸组织氧化损伤的影响。方法将4周雄性Wistar大鼠随机分为对照组、模型组、LBP组、LJP组和LBP+LJP联合组;灌胃7 d后进行连续4周(周六、周日除外)60Coγ照射,剂量2.3 Gy/只,照射期间每天灌胃;照射结束7d后进行组织切片观察睾丸形态改变,测定睾丸组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和还原型谷胱甘肽(GSH)、丙二醛(MDA)含量。结果对照组MDA含量为(1.30±0.27)nmol/mg,与模型组、LBP组、LJP组的(2.16±0.34),(1.86±0.29),(1.70±0.09)nmol/mg比较,差异均有统计学意义(P<0.01或P<0.05);LBP+LJP联合组MDA含量为(0.82±0.34)nmol/mg,与模型组比较,差异有统计学意义(P<0.01);对照组SOD活力为(155.11±4.49)U/mg,与模型组、LBP组、LJP组的(123.98±11.38),(137.60±7.38),(133.54±8.89)U/mg比较,差异均有统计学意义(P<0.01)。结论 LBP+LJP联合作用对电离辐射致睾丸组织损伤具有明显的保护作用,其效果优于LBP、LJP单独作用。

Objective To study the combined effect of the Laminaria japonica polysaccharides（LJP） and Lycium barbarum polysaccharides（LBP） on male rats＇ testicular tissue oxidative damage induced by chronic local ionizing radiation. Methods Four weeks old male Wistar rats were randomly divided into the control,model,LBP,LJP,and LBP＋LJP group.After one week adaptable rearing,apart from the control group,all groups were exposed to 60Coγ-ray local irradiation everyday for 20 days in four weeks（except the weekend） at a total radiation dose of 2.3 Gy for each rat.During the radiation,the rats were given intragastral administration according to the grouping everyday.Seven days after the cessation of exposure,the testicular tissue was observed and the levels of superoxide dismutase（SOD）,glutathione（GSH）,glutathione peroxidase（GSH-PX） and content of malonodialdehyde（MDA） in testicular tissue were determined. Results Testicular tissue in LBP ＋ LJP was normal.LBP ＋ LJP increased the contents of SOD,GSH-Px,GSH（P0.01） and reduced MDA content（P0.01）. Conclusion The radioprotective effects of combined LBP and LJP on testicular tissue are obvious and the combined effect is better than LBP or LJP independently.