假丝酵母菌1070株对3种药物药敏分析

Susceptibility of 1070 clinical isolates of Candida species to three antifungal agents

目的分析假丝酵母菌临床分离株的流行病学及其耐药性特点,为临床合理选择药物提供依据。方法采用CHROMagar显色培养基和Vitek2 YST鉴定条进行菌株鉴定,根据美国国家临床试验标准化研究所(CLSI)推荐的《酵母菌的液基稀释法抗真菌药物敏感试验参考方案第二版》(M27-A2)方案进行药敏试验。结果共收集假丝酵母菌1 070株,其中白假丝酵母菌最常见,占69.9%,其次为光滑假丝酵母菌和热带假丝酵母菌;假丝酵母菌对氟康唑的敏感率为87.8%,对5-氟胞嘧啶的敏感率为99.5%,对伊曲康唑的敏感率为40.3%。结论药敏结果显示假丝酵母菌临床分离株对5-氟胞嘧啶有高度的敏感性,而对伊曲康唑的耐药率很高。

Objective To evaluate susceptibilities of Candida species to three antifungal agents. Methods The Candida species isolates were differentiated by CHROMagar and Vitek2 YST test kits.And the susceptibility testing was performed with broth microdilution method according to the Clinical and Laboratory Standards Institute（CLSI）M27-A2 reference method for broth antifungal susceptibility testing of yeasts. Results Of the 1070 isolates collected,Candida albicans was the most frequently isolated species（748,69.90%）,followed by Candida glabrata and Candida tropicalis.Overall,87.8% of all Candida isolates were susceptible to fluconazole,99.5% to 5-fluorocytosine,and 40.3% to itraconazole. Conclusion All the Candida isolates are highly sensitive to 5-fluorocytosine.However,the resistant rate of all the Candida strains to itraconazole is high.