内关穴注射香丹注射液预处理对大鼠急性心肌缺血冠状动脉血管壁内皮上t-PA、PAI-1活性的影响

The Effects of Pretreatment Acupoint Injection of Xiangdan Parenteral Solution at Neiguan(PC6) on fibrinolytic system t-PA、PAI-1 in Acute Myocardial Ischemia Rats

目的:通过内关穴注射香丹注射液预处理对大鼠急性心肌缺血纤溶系统t-PA、PAI-1的影响,探讨针药结合预防和改善心肌缺血的作用机制。方法:以SD大鼠为受试对象,心肌缺血前给予内关穴注射香丹注射液预处理14天,同时设药物对照组和穴位注射组进行比较;用异丙肾上腺素腹腔皮下注射造成心肌缺血模型,取心脏进行心肌病理形态学观察,免疫组织化学半定量检查冠状动脉血管壁内皮上t-PA,PAI-1蛋白的含量。结果:内关穴位注射香丹注射液预处理方法通过升高冠状血管壁内皮上的t-PA含量,降低PAI-1含量来恢复纤溶系统的活性,减轻心肌细胞的损伤。并且针药组明显优于药物组与穴位组。结论:穴位注射预处理可以改善心肌的病形态,通过升高冠脉局部t-PA含量,降低PAI-1含量,从而减轻心肌组织在急性心肌缺血时的损伤。

Objectives：Through injecting HongDan parenteral solution into Neiguan point of acute myocardial ischemia rats to understand how HongDan parenteral solution working on t-PA、PAI-1 activity of endothelium-dependent＇s fibrinolytic system.And to discuss the influence on fibrinolytic activity.Methods：SD rats were pretreated for 14 days by injection of HongDan parenteral solution into Neiguan point,before copying ischemia myocardial model.and compare with the Acupoint injection group and the medicine group.Then the ischemia myocardial model was copyed by peritoneal subcutaneous injecting isoproterenol（ISO）.The myocardium pathomorphology was observed,the t-PA,PAI-1 protein of coronary artery blood vessel endothelium were detected by immunohistochemisty.Conclusions：Pretreatment of injecting HongDan parenteral solution into Neiguan point can raise the contents of t-PA and decrease the contents of PAI-1,and prevent the cardiac muscle of acute myocardial ischemia rats.