细胞分裂素对吊兰根生长和负向光性的影响

Effects of cytokinin on the growth and negative phototropism of Chlorophytum comosum var.variegata root

为了研究细胞分裂素对吊兰根生长和负向光性的影响,采用水培法将吊兰培养在透明玻璃缸内,并向水培液中添加6-BA和对水培吊兰根系进行单侧照光的方法,研究结果表明:(1)6-BA对吊兰不定根和侧根的生长以及负向光性都有抑制作用,抑制程度随着浓度的增加而加剧;(2)10mg·L^(-1)6-BA处理7d后和100mg·L^(-1)6-BA 5天后不定根停止生长;(3)10-100 mg·L^(-1)6-BA处理的吊兰侧根均不能伸长,只能够在不定根表明形成凸起的结构,但凸起的数量随着6-BA浓度的增加而增加;(4)吊兰根系在无6-BA处理时在单侧光下弯曲度可达到接近90°,100 mg·L^(-1)6-BA可使其弯曲度下降至15°左右。探讨了6-BA抑制吊兰根生长和负向光性的可能机理。

To explore the effects of cytokinin on the growth and negative phototropism of Chlorophytum comosum var. variegata root, some healthy plants of Chlorophytum comosum var. variegata were selected and cultivated in different concentration of 6-BA solution with unilateral light illuminated, and the growth rate and the negative inclination of the root of chlorophytum comosum were measured. The results showed that： （1） 6-BA inhibited the growth of both the adventitious root and the branch root growing out from the adventitious root, decreased the bend angle of the negative phototropism of all of the elongated root, the inhibited degree was intensified with the increase of 6-BA concentration. （2）The adventitious root ceased to elongation when treated for 7 days with 10mg·L^-1 6-BA or for 5 days with100mg·L^-1 6-BA; （3） All the branch root growing out from the adventitious root could not extend but form much little bump from the surface of the adventitious root when treated with 10-100 mg·L^-1 6-BA, the amount of the bump from the surface of the adventitious root were increased with the rise the concentration of 6-BA; （4） The negative phototropism of control was remarkable and inclination angle was near 90° , but inclination angle decreased to about 15° when treated withl00mg·L^-1 6-BA. The possible mechanism was scussed in this study.