黄芪对动脉粥样硬化兔骨髓内皮祖细胞生长的影响

Effect of astragalus mongholicus on the growth of endothelial progenitor cells from bone marrow of atherosclerotic rabbits

目的:研究黄芪对冠状动脉粥样硬化模型兔骨髓内皮祖细胞(endothelial progenitor cells,EPCs)生长的影响,并探讨黄芪对冠心病临床治疗的可能机制。方法:建立动脉粥样硬化模型,分为正常对照组和动脉粥样硬化组,体外分离培养EPCs,通过荧光显微镜观察细胞经乙酰化低密度脂蛋白(DiI-ac-LDL)摄取试验和荆豆凝集素(FITC-UEA-I)和体外成血管试剂matrigel观察细胞成血管功能来鉴定EPCs,检测细胞黏附能力、迁移能力及增殖能力。结果:动脉粥样硬化组兔冠状动脉可见斑块或脂纹;DiI-ac-LDL和FITC-UEA-I双染色阳性;培养6d的EPCs可见血管腔样的结构;动脉粥样硬化组EPCs的数量、黏附、迁移及增殖能力较正常对照组明显下降,差异有显著性(P<0.05)。黄芪可以一定程度上促进动脉粥样硬化组兔骨髓EPCs的数量的增加及黏附、迁移和增殖能力,并以20g/L最为明显,差异有显著性(P<0.05)。结论:黄芪治疗冠心病的作用机制可能为促进EPCs的生长。

Objective To investigate the effect of astragalus mongholicus on the growth of endothelial progenitor cells from bone marrow of atherosclerotie rabbits, and to investigate the potential of astragalus for treatment of coronary heart disease. Methods New Zealand white rabbits were randomly divided into the control group （n = 6） and the athcrosclerosis group （n = 6） to establish animal models of atherosclerosis. EPCs were cultured and identified by DiI-Ac-LDL uptake and FITC-UEA-I-lectin binding using fluorescence microscopy （FM）. The ability of tube structure formation of EPCs were assessed using matrigel reagent kit in vitro. The adhesion, migration and proliferation ability of EPCs were also measured in these two groups. Results Plaques and fatty streak leisions, which were positive for DiI-ac-LDL-uptaking and FITC-UEA-I-lectin staining, could be observed in coronary arteries from rabbit models of atherosclerosis. Capillary formation could be observed 6 days post EPCs culturing. Compared with those in the control group, the cell number, the adhesion, migration and proliferation abilities of EPCs decreased significantly in the atherosclerosis group （P 〈 0.05 ）; Astragalus could significantly increase the EPCs number and the adhesion, migration and proliferation abilities of EPCs at 20 g/L （P 〈 0.05） than other dosage. Conclusion The potential mechanism for Astragalus as a treatment of coronary artery disease is its contribution to EPCs growth.