柚皮苷印迹β-环糊精聚合物的制备及性能研究

Preparation and Property Studies of Naringin Imprinting β-Cyclodextrin Polymer

以柚皮苷(NG)为印迹分子,β-环糊精为功能体,六亚甲基二异氰酸酯为交联剂,采用乳液聚合法制备了对NG具有特定识别能力的吸附材料:棒状印迹聚合物.扫描电镜及比表面分析仪测试结果表明印迹聚合物具有较大的孔隙及比表面积;红外及核磁共振谱研究证实了识别位点来自β-环糊精与NG羟基间的氢键作用.采用平衡吸附实验方法研究了聚合物的吸附性能和选择性能.实验结果表明,棒状印迹聚合物(RMⅠP)对NG具有较高的亲和性和选择性.Scatchard分析表明,MⅠP在识别NG过程中存在2类结合位点:KD1=0.016 mmol/L,Bmax1=15.31μmol/g,KD2=0.24 mmol/L,Bmax2=98.41μmol/g.当NG浓度为0.02 mg/mL时,MⅠP及相应NⅠP对NG的分配系数KD分别为4.38和2.86,印迹因子α为1.53.

Rod-like molecularly imprinted polymer（RMIP） was prepared by emulsion polymerization us-ing β-cyclodextrin（β-CD） as a functional polymer,naringin（NG） as a template molecule and hexamethyl-ene diisocyanate（HMDI） as crosslinker.The morphology,pore size distribution and specific surface area of the RMIP were observed by means of scanning electron microscopy（SEM） and BET adsorption apparatus.The interaction between the template molecule and functional polymer was analyzed by the infrared spec-trum（IR） and 1H-nuclear magnetic resonance（1H NMR）.Its adsorption property and selectivity were evalu-ated by equilibrium adsorption experiment method.The obtained results revealed appropriate affinity and selectivity of RMIP to NG.Scatchard analysis suggests that during RMIP recognition of NG,there were two classes of binding sites.The calculated dissociation constant KD1 and apparent maximum number Bmax1 of binding sites with high affinity were 0.016 mmol/L and 15.31 μmol/g respectively,while KD2 and Bmax2 of binding sites with low affinity were 0.24 mmol/L and 98.41 μmol/g.In 0.02 mg/mL NG solution,the dis-tribution coefficients KD of NG on RMIP and corresponding NIP are 4.38 and 2.86,respectively.The im-printing factor α is 1.53.