摘要
目的探讨乙酰化对人神经母细胞瘤SK-N-SH细胞中NOS1基因启动子活性的调节作用。方法构建系列截短的NOS1 5′侧翼序列萤光素酶报告基因载体,应用萤光素酶报告基因检测系统分析SK-N-SH细胞中NOS1启动子活性,并比较组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)刺激前后启动子活性的变化。结果构建并鉴定了系列截短的NOS1启动子萤光素酶报告基因载体;在NOS1基因启动子区鉴定了两个正调控区域和两个负调控区域,同时TSA可上调不同长度启动子的转录活性。结论乙酰化参与调节神经细胞中NOS1基因启动子活性。
Objective To assess the regulation of NOS1 promoter activity by acetylation in human neuroblastoma SK-N-SH cells.Methods A series of luciferase reporter vectors of truncated NOS1 5′ flanking region were constructed.NOS1 promoter activity in SK-N-SH cells was analyzed by using luciferase reporter system,and the change in the promoter activity in SK-N-SH cells exposed to Trichostatin A(TSA),a histone deacetylase inhibitor,was detected.Results A series of luciferase reporter vectors of truncated NOS1 promoter were constructed and identified.For NOS1 promoter,2 positive regulatory regions and 2 negative regulatory regions were found.TSA up-regulated the transcription activities of NOS1 promoters of different lengths.Conclusion Acetylation may participate in the regulation of NOS1 promoter activity in neuronal cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第3期204-205,209,共3页
Journal of China Medical University
基金
辽宁省教育厅高校科研基金计划项目(2008858)
国家自然科学基金资助项目(30900807)