大鼠局灶性脑缺血再灌注后BDNF mRNA及其蛋白的表达变化

Changes of brain-derived neurotrophic factor mRNA and protein expression in the penumbra of rat cortex after focal cerebral ischemia/reperfusion

目的观察大鼠脑缺血再灌注后缺血半暗带皮质脑源性神经营养因子(BDNF)mRNA和蛋白的表达变化。方法采用线栓法制作局灶性脑缺血大鼠(MCAO)模型,用原位杂交法和免疫组化法观察脑缺血后3、6、12h及1、3、7d共6个时间点BDNF mRNA及其蛋白的表达变化。结果缺血半暗带BDNF mRNA及其蛋白的表达均于缺血再灌注后3h开始明显上升,6h表达继续增强,12h达高峰,3d后表达开始减弱(P<0.05或0.01),7d后降至基础水平。结论脑缺血再灌注后缺血半暗带BDNFmRNA及蛋白表达均明显增加,提示其可能参与了脑缺血后神经保护。

Objective To observe the mRNA and protein levels of brain-derived neurotrophic factor（BDNF） in the penumbra of rat cortex after focal cerebral ischemia/reperfusion（I/R）. Methods The I/R rat models were established by middle cerebral artery occlusion（MCAO） method to observe the expression variation of BDNF mRNA and protein at 3,6, 12h and 1,4,7d after I/R by in situ hybridization and immunochemistry method. Results The expressions of BDNF mR- NA and protein in the penumbra region increased at 3h after reperfusion, and reached the peak at 12h, then decreased con- tinuously after 3d （ P 〈 0. 05 or 0.01 ）, and decreased to nomal level after 7d. Conclusion The mRNA and protein levels of BDNF increase in the penumbra of rat cortex after focal cerebral ischemia which suggests that it may protect neurons from apoptosis following cerebral ischemia.