HPLC法同时测定救心丸中华蟾酥毒基、脂蟾毒配基含量

Content determination of cinobufagin and resibufogenin in Jiuxin Pills by HPLC

目的:建立救心丸中华蟾酥毒基、脂蟾毒配基的含量测定方法。方法:色谱柱:C18(250 mm×4.6 mm,5μm);柱温:40℃;流动相:乙腈-0.5%磷酸二氢钾(pH=3.2)(50∶50);流速:1.0 ml/min;检测波长:296 nm。结果:华蟾酥毒基在0.498～2.490μg范围内线性关系良好;脂蟾毒配基在0.570～2.850μg范围内线性关系良好。结论:本方法简便、准确,重复性好,可作为救心丸的质量控制方法。

Objective：To establish an HPLC method for content determination of cinobufagin and resibufogenin in Jiuxin Pills.Methods：The column was C18（250 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-0.5%KH2PO4（pH=3.2）（50∶50）and the flow rate was 1.0 ml/min.The detection wavelength was at 296 nm.Results：Cinobufagin had a good linearity between 0.498-2.490 μg and resibufogenin had a good linearity between 0.570-2.850 μg.Conclusion：The method is simple and accurate with good repeatability and can be used for the quality control of cinobufagin and resibufogenin in Jiuxin Pills.