复方利福平片的HPLC测定法

Study on Quantitative Determination of Co m pound Rifa m picin Tablets

采用 Ｈ Ｐ Ｌ Ｃ 法，以 Ｗａｔｅｒｓ μ Ｐｏｒａｓｉｌ 为固定相，异丙醇甲醇０ ．０５ ｍｏｌ Ｌ 磷酸盐缓冲液（５６∶４２∶２ ，ｐ Ｈ＝７ ．０） 为流动相，在２５４ ｎｍ 波长处，同时测定复方利福平片中利福平、异烟肼和吡嗪酰胺的含量。

This paperreportsthe quantitative determination ofrifampicin ,isoniazid and pyrazinamidein the compoundrifampicin tablets by H P L Cat wavelength of 254 nm . The stationary phase was Waters μ Porasil column . The mobilephase was composed ofisopropanol methanol phosphate buffer (56∶42∶2) . The average recoveries were 99 .05 % ～100 .08 % .