RP-HPLC法测定不同产地及采收期的穿心莲中穿心莲内酯、新穿心莲内酯的含量

R P H P L C Determination of Andrographolide and Neoandrogra pholide in Andrographis paniculata Nees of Different Places and Collected Tim es

采用反相高效液相色谱法测定穿心莲中穿心莲内酯、新穿心莲内酯的含量。色谱柱为 Ｍｅｒｃｋ Ｌｉｃｈｒｏｍａ Ｒ Ｐ Ｃ１８ 柱，流动相为乙腈水（３５∶６５） ，检测波长为２２５ ｎｍ ，流速为１ ｍｌｍｉｎ ，柱温４０ ℃，穿心莲内酯和新穿心莲内酯的加样回收率分别为１０２ ．６２ ％ 、９８ ．６ ％ ， Ｒ Ｓ Ｄ 分别为０ ．４８ ％ 、０ ．４７ ％ ，上述两成分在广东阳春和安徽临泉所产的穿心莲中含量较高，最佳采收期为开花盛期。

The contents of andrographolide and neoandrographolide contained in Andrographis paniculata Nees . wereanalysed by reverse phase H P L Cusing a Merck Lichroma R P C1 8 column with acetonitrile water(35∶65) asthe mobilephase. The recoveries for andrographolide and neoandrographolide were 102 .62 % and 98 .6 % , respectively . Thehighest contents of andrographolide and neoandrographolide are found in the samples cultivated in Yangchun , Guangdong ,and Linquan , Anhui . The best collecting time is on flower blossoming . The leaf showed the highercontent than stem . Our analysis also indicated that andrographolide and neoandrographolide decompose quickly on longtime of storage .