人子宫内膜细胞体外培养技术的改良研究

Improvement of Method for Human Endometrial Cell Culture in Vitro

目的:通过体外分离培养纯化子宫内膜间质细胞和腺上皮细胞,建立原代子宫内膜间质细胞和腺上皮细胞模型。方法:酶解新鲜子宫内膜组织,经过反复过滤、离心、细胞传代,得到子宫内膜间质细胞和腺上皮细胞。用免疫细胞化学检测其纯度。结果:分离的间质细胞纯度可达94%以上,间质细胞可有限传代,并至少可存活1月余;分离的腺上皮细胞纯度可达90%以上,腺上皮细胞不能长久生长。结论:建立了可体外培养、传代的子宫内膜细胞。该方法可在分子水平研究子宫内膜细胞的功能,且省时、高效。

Objective：To establish a primary endometrial glandular epithelial and stromal cells model from the method of isolation 、purifying and culturing from human endometrial specimens.Methods：Enzymolysis the fresh human endometrial tissue,and filter、centrifugate、passage it,to obtain endometrial glandular epithelial and stromal cells.And detect the purity by immunocytochemistry method.Results：The purities of stromal cells were 94%,they could be reproduced into several passage,and could be survive for more than one month;the purities of glandular cells were 90%,but they couldn＇t be survive longly.Conclusion：The method could be used to establish the model of endometrial cell which could be cultured、reproduced in vitro,it is a shortcut、high performance method,and it can help study their function and molecular regulation.