RP-HPLC测定扑热息痛颗粒剂中药物及其人血浆中药动学研究

Determination of Aceta minophen in Hu m an Plas m by R P H P L C and Its Pharm acokinetics

Areversed phase liquid chromatographic method for determination of acetaminophen in human plasm andthe pharmacokinetics ofacetaminophen in humans were studied . The drug was extracted from human plasm with diethylether . A C1 8 column (5 μm ,200 m m ×4 .6 m m ) accompaned with the mobil phase composed of methanol 0 .022 mol L Na Ac 0 .126 mol L H Ac (25 ∶50 ∶50) was used . Salicylic acid was used as internal standard and detectivewavelength was 233 nm . The calibration curves showed good linearity overthe range of 0 .112 μgml. The recoveries ofacetaminophen were 94 .3 % 101 .7 % for plasma . After a single dose of 500 mg of acetaminophen granula , theconcentration time curves conformed to one compartment model. Cm a x = (5 .94 ±0 .69) μgml , tm ax = (0 .73 ±0 .32)h ,t1 2 Ke = (2 .38 ±0 .85) h , t12 Ka = (0 .21 ±0 .14 ) h , A U C0 → ∞ = (25 .87 ±6 .73) μg·h ml .