含有Ⅰ、Ⅳ型胶原的静脉管腔对周围神经缺损修复作用的影响

The venous segments contained collagen Ⅰ and Ⅳ effect on the repair of peripheral nerve defects

目的为弄清楚含不同类型胶原的静脉管腔在修复周围神经缺损中的作用。方法将Ⅰ、Ⅳ型胶原注入静脉管腔内用于桥接兔腓总神经１．５ｃｍ长缺损，以生理盐水作为对照组，术后１６周进行电生理、组织形态学和有髓纤维计数等项检查。结果Ⅰ型胶原组与生理盐水对照组各项检测指标间差异无显著性；Ⅳ型胶原神经传导速度和肌肉动作电位的恢复情况均优于Ⅰ型胶原组，有髓纤维计数两组间差异无显著性。与生理盐水组相比较，Ⅳ型胶原组仅神经传导速度恢复程度较好，其它各项指标检测差异无显著性。结论Ⅰ型胶原用于静脉桥接体内对周围神经缺损的修复无明显的促进作用；Ⅳ型胶原虽优于Ⅰ型胶原，但该种促进作用的表现并不十分显著。

Objective To studies the effects of collagen Ⅰ and Ⅳ which were injected into the bridge material of venous segments on the repair of peripheral nerve defects Methods 1 5 cm long rabbit's peroneal nerve defects were made and the defects were bridged with autogenous venous segments The animals were divided into three groups In the first and second groups, 25 μg type Ⅰ and type Ⅳ collagen were injected into each group's venous segments separately In the third group (control group), 40 μl saline was injected The examination methods include: electrophysiological,histological examination and the counts of myelinated axons 16 weeks after operation Results The electrophysiological examination showed that the nerve conduction velocity and muscle action potentital of type Ⅳ collagen group recovered better than that of the type Ⅰ collagen group There is not sufficient difference between the type Ⅰ collagen group and control group The nerve conduction velocity recovered better in type Ⅳ collagen group than that in control group, and the muscle action potentials in those two groups were not significant difference The numbers of myelinated axons of three groups were not sufficient difference Conclusion Type Ⅰ collagen injected into the venous segments to bridge nerve defects has not support effects on peripheral nerve repair The effects of type Ⅳ collagen are better than that of type Ⅰ collagen, but those are not remarkable