165例小儿肾穿刺组织免疫荧光病理检查技术分析

Pathological Immunofluorescence Study on 165 Cases of Renal Biopsy in Children

目的探讨肾穿刺技术对小儿肾病诊断上的价值。方法对165例小儿肾病在B超引导下经皮肾穿刺活检,石蜡切片和冰冻切片,石蜡切片分别做HE、PAS、PASM、Masson染色,冰冻切片分别做IgG、IgM、IgA、C3、C1q及Fibrinogen染色。结果石蜡切片的4种染色对比鲜明,各种病理改变清晰,肾小球数量充足≥10个。冰冻切片免疫荧光染色背景清晰,肾小球数量均≥5个,没有冰晶形成,荧光标记明确,所有病种可分别在病变相应部位找到团块状、线状或颗粒状的黄绿色荧光。结论通过改进标本的处理方法能提高制片质量,明显提高肾病的诊断率。

Objective To investigate diagnostic value of the renal biopsy in children with kidney disease. Methods For ultrasound guided percutaneous renal biopsy, Both paraffin and frozen specimens were inspected. Paraffin sections were done for HE,PAS,PASM,and Massan staining,and the frozen sections were prepared for IgG, IgM,IgA,C3 and C1q. The method of sample processing was modified to improve production quality（ Cuting up,Fixing, Dyeing,etc）. Results On the paraffin sections with the four staining methods,a variety of pathological changes was observed with contrast clearly,and a sufficient number of glomeruli over ten was seen on each section. The number of glomeruli over five was observed on immunofluorescence staining of frozen section with clear background. No ice crystals formed, and the fluorescent marker was clear. Positive parts of Imnps or linear or granular yellow-green fluorescence staining were seen at the glomerular lesion sites corresponding to the type of the kidney diseases. Conclusion A diagnosis may be made by analyzing the various results with significant improvement in the diagnostic rate of different kidney diseases.