基于油茶组59万条EST序列的转录组学初步分析

Transcriptome Characterization for Camellia Sect.Oleifera Based on the 592 499 ESTs

油茶组（Camellia Sect.Oleifera）植物是我国特有的木本油料植物,主要分布全国14个省（区）,种植面积达300万hm2。茶油为油茶组多个树种种仁油的统称,其中以油茶（Camellia oleifera）最为常见,其次是浙江红山茶（C.chekiangoleosa）和短柱茶（C.brevistyla）等。茶油是优质食用植物油,富含油酸、亚油酸等不饱和脂肪酸,且易消化耐贮藏（何方等,2004）。大幅提高茶油在食用油供给中的比例,可以缓解我国日益紧张的食用油供给矛盾。

To investigate gene diversity and expression pattern in Camellia Sect.Oleifera,454 GS FLX platform and Titanium regent were used to produce expressed sequence tags（ESTs） from flower buds of 3 Camellia species.A total of 5 920 499 ESTs were generated.Clustering those sequences twice by Cap3 produced 15 733 contigs and 20 606 singlets in C.oleifera,19 397 contigs and 26 882 singlets in C.chekiangoleosa,14 779 contigs and 25 389 singlets in C.brevistyla.The GC gradients in 3 transcriptomes basically followed a normal distribution.In additional all contigs and singlets were functionally categorized into GO（Gene Ontology） categories respectively.