摘要
目的探讨跨膜丝氨酸蛋白酶4(transmembrane protease serine 4,TMPRSS4)和癌胚纤维连接蛋白(oncofe-tal fibronectin,onfFN)在细针吸细胞学检查(fine-needle aspiration biopsies,FNAB)标本中的定量表达。方法采用荧光定量逆转录-聚合酶链反应(RT-PCR),检测细针吸组织中TMPRSS4和onfFN mRNA的表达量,比较良恶性组织中2种分子标记物的表达差异。结果甲状腺癌和甲状腺良性病变组织中TMPRSS4和onfFN mRNA的平均表达量分别为:15.236±9.6850、.569±0.374及25.318±15.642、0.865±0.219,恶性组织与良性组织中两者表达差异具有统计学意义(P均=0.000),甲状腺癌组织中2种分子标志物的表达呈正相关(γ=0.374,P=0.026)。2种分子标记物表达与患者性别、年龄、肿瘤大小无相关性。TMPRSS4 mRNA和onfFN mRNA表达与肿瘤TNM分期和淋巴结转移密切相关(P=0.009、P=0.015,P=0.024、P=0.08)。结论 TMPRSS4 mRNA和onfFN mRNA在甲状腺癌组织中表达水平上调,两者与细针吸细胞学检查联合,对甲状腺病变的诊断和鉴别诊断具有很高的应用价值。同时,两者与甲状腺癌的发展密切相关,而且两者可能具有协同作用。
Objective To study the expression of Transmembrane Protease Serine4（TMPRSS4） and Oncofetal Fibronectins（onfFN） in the thyroid nodule sample of FNA.Methods The expression quantity of TMPRSS4 and onfFN mRNA was detected,and the expression differences between the tissues from the carcinoma and adenoma were compared.Results The expression quantity of TMPRSS4 in carcinoma and adenoma tissue was 15.236±9.685,0.569±0.374 respectively,and onfFN was 25.318±15.642,0.865±0.219 respectively.There was a statistically significant higher expression of thyroid carcinoma than adenoma（P=0.000）,and there was a statistically significant positive correlation between expression of TMPRSS4 and onfFN（γ=0.374,P=0.026）.The expression of TMPRSS4 and onfFN mRNA in TNM Ⅲ＋Ⅳand lymph node metastasis was higher than TNM Ⅰ＋Ⅱand no lymph node metastasis thyroid carcinoma samples（P=0.009,P=0.015 and P=0.024,P=0.08）,but,There was no statistically significant in other clinicopathologic parameters.Conclusion The mRNA of TMPRSS4 and onfFN are up-regulated in thyroid carcinoma,it might be useful in the diagnoses and differential diagnoses of thyroid lesions if combined FNA with the detection of the TMPRSS4 and onfFN mRNA.The two markers are positively correlated and might have synergistic effect in the development of the thyroid carcinoma.
出处
《实用癌症杂志》
2011年第2期144-146,共3页
The Practical Journal of Cancer
