PI3K p110α在宫颈病变中的表达研究

Study of PI3K p110α Expression in Cervical Lesions

目的研究PI3K p110α蛋白在宫颈上皮内瘤变和宫颈癌中的表达及其与临床病理特征的关系。方法应用ElivisionTMSuper免疫组化法检测40例正常宫颈、31例CINⅠ、33例CINⅡ、35例CINⅢ和41例宫颈癌石蜡标本中PI3K p110α的表达,比较各组间的差别及与患者临床病理特征的关系。结果①PI3K p110α在正常宫颈、CINⅠ、CINⅡ、CINⅢ及宫颈癌中的阳性率依次为0.0%、19.4%、57.6%、65.7%、80.5%。②比较正常宫颈与CINⅠ之间(χ2=8.451,P=0.004)及CINⅠ与CINⅡ之间(χ2=9.810,P=0.002),PI3K p110α阳性率差异有统计学意义;CINⅡ组与CINⅢ组之间(χ2=0.476,P=0.490)及CINⅢ与宫颈癌之间(χ2=2.125,P=0.145)比较,随着分期的增加呈上升趋势,但差异无统计学意义。③PI3K p110α在LSIL和HSIL中的阳性率分别是19.4%和61.8%,差异有统计学意义(χ2=15.333,P=0.000),可将其视为宫颈LSIL与HSIL间的辅助鉴别指标。④PI3K p110α蛋白表达与宫颈癌临床分期、组织分化程度及有无淋巴结转移有关(P<0.05)。结论 PI3K p110α在除正常宫颈组织外的各级宫颈病变组织中均呈阳性表达,抑制其活性可能为宫颈癌的治疗提供新靶点。

Objective To study the clinical pathological significance of PI3K p110α expression in cervical cancer（CC） and cervical intraeptithelial neoplasia （CIN）. Methods We detected the expression of PI3K p110α in 40 cases of normal cervix,31 cases of CINⅠ,33 cases of CINⅡ,35 cases of CINⅢ and 41 cases of CC by ElivisionTM Super immunohistochemistry,and analyzed the relationship between the expression and clinicopathological characteristics. Results The positive ratio of PI3K p110α in normal cervix,CINⅠ,CINⅡ,CINⅢ and CC was 0.0%,19.4%,57.6%,65.7% and 80.5%,respectively.There were statistically significant differences in the positive ratio of PI3K p110α expression between normal cervical epithelial tissue and CINⅠ（χ2=8.451,P=0.004）,also between CIN I and CINⅡ（χ2=9.810,P=0.002）.No statistically significant difference was found in the positive ratio of PI3K p110α expression between CIN Ⅱ and CIN Ⅲ（χ2=0.476,P=0.490）,also between CIN Ⅲ and cervical cancer（χ2=2.125,P=0.145）,however,the PI3K p110α expression showed an ascending tendency with the increasing histological stage.Positive ratio of PI3K p110α in LSIL and HSIL was 19.4% and 61.8% respectively,and the difference was statistically significant（χ2=15.333,P=0.000）.PI3K p110α may be a good ancillary index to screen HSIL of cervix.There was a positive correlation between PI3K p110α expression and clinicopathological characteristics. Conclusions Excepting normal cervical tissue,PI3K p110α expression is positive in all stages of cervical lesions.Inhibiting PI3K p110α activity may provide a new target for cervical cancer treatment.