基因型为杂合子的氟烷阳性猪检测

DETECTION OF HALOTHANE GENOTYPE WITH PCR RFLP AND DISCOVERY OF HALOTHANE POSITIVE PIG WITH HETEROZYGOTE

对１４７ 头猪进行了氟烷测验，测得氟烷阳性猪３ 头，阳性率为 ２．０４％ 。运用 Ｐ Ｃ Ｒ Ｒ Ｆ Ｌ Ｐ分析法对氟烷阳性猪及其同胞进行了基因型检测，结果４ 头被测猪中有２ 头为隐性纯合子，２ 头为杂合子。结合氟烷测验结果，２ 头隐性纯合子表现为氟烷阳性，符合一般规律；而２ 头杂合子中发现１ 头为氟烷阳性，此前尚未见报道，这一结果又一次证明了氟烷表现与基因型并非完全对应。

Halothane test(HT) was carried out on 147 pigs.3 pigs were halothane positive (HP),HP rate was 2.04%.The genotypes of HP pigs and their siblings(total 4 pigs)were detected with PCR RFLP.Two of them were recessive homozygote (nn) pigs,and the other 2 were heterozygote (Nn)pigs.In comparison with the results of HT and PCR RFLP,it was showed that 2 nn pigs were HP,which accorded with the general regularity and that 1 of 2 Nn pigs was found to be HP,which had not been reported before.Therefore,it was proved again that there was no complete corresponding relationship between halothane phenotype and genotype.