Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响

Effects of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells

目的探讨Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响及机制。方法以Hep2细胞为对照组,转染pcDNA3.1-Mst1的Hep2细胞为转染组,SB203580处理的Mst1转染细胞为处理组。采用MTT法测定三组细胞的增殖抑制率,双标流式细胞法检测其凋亡率,用Western blot法检测三组细胞中Mst1蛋白、磷酸化p38(p-p38)和总p38蛋白(t-p38)的表达情况。结果与对照组相比,转染组Mst1和p-p38蛋白表达增强明显t,-p38的表达无明显变化,凋亡率由2.46%升高至7.37%,细胞增殖抑制率为41.8%。与转染组相比,处理组p-p38蛋白表达增强降低t,-p38表达无明显变化,细胞凋亡率由7.37%降低至2.65%,细胞抑制率为-10.1%,细胞增殖活力显著降低。结论 Mst1基因能够通过p38 MAPK通路参与喉癌细胞凋亡和增殖的调控。

Objective To investigate the influence and mechanism of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells.Methods The Hep2 cells were regarded as control group,and the Hep2 cells transfected with pcDNA3.1-Mst1 as Mst1 transfected group,the Mst1 transfected group cells treated with SB203580 as SB203580 treated group.In the three groups,the inhibitory rate of cell growth was detected by MTT,the apoptosis was detected by flow cytometry,and the protein expression of Mst1 and phosphorylated p38（p-p38） and total p38（t-p38） were detected by Western blot.Results Compared with control group,the protein expression of Mst1 and p-p38 in Mst1 transfected group up-regulated significantly,t-p38 protein showed no changes,the apoptosis radio increased from 2.46% to 7.37%,the inhibitory rate of cell growth was 41.8%.Compared with Mst1 transfected group,the protein expression of p-p38 in SB203580 treated group down-regulated significantly,t-p38 protein showed no changes,the apoptosis radio decreased from 7.37% to 2.65%,the inhibitory rate of cell growth was-10.1%,cell proliferation decreased significantly.Conclusion Mst1 gene can regulate the apoptosis and proliferation of laryngeal carcinoma cells through p38 MAPK pathway.