骨关节炎与Asporin基因多态性关联性的Meta分析

Meta-analysis of association between Asporin polymorphism and osteoarthritis

背景:流行病学研究表明,骨关节炎具有较强的遗传倾向,且之前的一些研究己经确定并证实了存在骨关节炎相关易感基因。在希腊、英国、西班牙、中国和韩国也有关于骨关节炎与Asporin基因多态性关联性的研究,但得出的结论不一致。目的:采用Meta分析评价骨关节炎与Asporin基因多态性的关联性。方法:检索PubMed、EMBASE、Cochrane图书馆及万方、维普和中国生物医学文献数据库2000-01/2010-07有关骨关节炎病例对照研究的论著文章,文献中等位基因分布频率符合Hardy-Weinberg(H-w)遗传平衡定律。以"osteoarthritis,Asporin"为英文关键词,以"骨关节炎,Asporin,基因"为中文关键词,共检索到48篇文献。应用采用Cochrane协作网提供的RevMan5.0对各研究结果进行异质性检验和效应值合并。结果与结论:总计纳入文献6篇,共有膝关节骨关节炎患者1560例,对照组2672例;髋关节骨关节炎患者1800例,对照组1416例。在膝关节骨关节炎患者中,总研究人群中D14/其他所有基因的合并效应量OR值=1.23,95%CI为(0.94～1.61),P>0.05;D13/其他所有基因的合并效应量OR值=0.90,95%CI为(0.76～1.06),P>0.05。分层分析显示,在亚洲组D14/其他所有基因和D13/其他所有基因,P均>0.05;在欧洲组D14/其他所有基因,P>0.05;D13/其他所有基因合并效应量OR值=0.83和95%CI为(0.72～0.95),P<0.05。在髋关节骨关节炎中,D14/其他所有基因和D13/其他所有基因的P均>0.05。总体来说髋、膝关节骨关节炎与Asporin基因无关联。但在欧洲人群D13与膝关节骨关节炎的发生有关联,Asporin基因多态性中的D13等位基因在欧洲人群的膝关节骨关节炎患者中可能是一种保护基因。

BACKGROUND：Epidemiologic studies have shown that osteoarthritis has genetic predisposition.Osteoarthritis-related predisposing genes have been discovered.There are studies focusing on osteoarthritis and Asporin polymorphism in Greece,UK,Spain,China and Korea.However,the results vary.OBJECTIVE：To assess the association between Asporin polymorphism and osteoarthritis using Meta analysis.METHODS：Related articles were searched from PubMed,EMBASE,Cochrane library in English and from Wanfang,VIP,CBM in Chinese published by from January 2000 to July 2010,with key words ＂osteoarthritis,Asporin＂ in English and ＂osteoarthritis,Asporin,gene＂ in Chinese.A total of 48 relevant articles were collected which met the criteria and Hardy-Weinberg（H-w） genetic laws.The correlated index was extracted for analysis in RevMan 5.0.RESULTS AND CONCLUSION：Finally,6 were included,involving 1 560 patients with knee osteoarthritis and 2 672 controls;1 800 patients with hip osteoarthritis and 1 416 controls.Of knee osteoarthritis patients,D14/others genotype exhibited OR value=1.23,95% CI （0.94-1.61）,P0.05;and D13/others：OR=0.90,95%CI （0.76-1.06）,P0.05.Subgroup analysis showed that the P of D14/others and D13/others0.05 in Asian group,while in European group the P of 14/others0.05;the OR values of D13/others were 0.83,95%CI （0.72-0.95）,P0.05.Of hip osteoarthritis patients,D14/others and D13/others exhibited P values0.05.Results suggest no association between Asporin and knee or hip osteoarthritis,but the recessive protective effects were observed for the D13 allele in knee European populations.