摘要
通过5种限制性内切酶获得了13匹德国骑乘马线粒体DNA(mtDNA)的切割图谱。结果发现,限制性消化产生了1(EcoRⅠ),3(BglⅡ),4(BamHⅠ)或5个(HindⅢ和BamHⅠ)限制性片断,并确定了所有片断的分子大小。mtDNA总的大小平均为(16.48±0.10)kb.除BamHⅠ外,mtDNA的限制性切点对于所有的个体是完全一致的。用BamHⅠ检测到mtDNA的多态性,通过带谱分析推断,德国骑乘马的B型mtDNA是由A型mtDNA通过突变产生1个新的切割位点进化而来。
German saddle horses cleavage patterns of mtDNA from 5 restriction endonucleases ( Bam HⅠ, Hin dⅢ, Eco RⅠ, Eco R V and Bgl Ⅱ) were obtained.The restriction digests yielded one ( Eco R Ⅴ ),3 ( Bgl Ⅱ),4 ( Bam HⅠ) or 5 ( Hin dⅢ and Bam HⅠ) fragments and all fragment sizes were given.The mean total size of mtDNA was calculated to be (16.48±0.10) kilobases.The restriction cleavage sites of mtDNA for all individuals except Bam HⅠ were completely identical.A polymorphism of mtDNA was detected with Bam HⅠ.The kind of polymorphism let us assume that B type mtDNA of this horse had evolved from the A type mtDNA by acquiring an additional cleavage site.
出处
《西北农业大学学报》
CSCD
北大核心
1999年第4期23-27,共5页
Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)
基金
德国萨尔州自然科学基金