新疆棉铃虫性信息素的电生理研究

THE ELECTROPHYSIOLOGICAL STUDIES ON THE SEX PHEROMONE OF THE COTTON BOLLWORM IN XINJIANG

以新疆喀什和库尔勒的棉铃虫为研究对象，利用触角电位记录技术，测定棉铃虫对标准化合物Ｚ１１１６：ＡＬＤ、Ｚ９１６：ＡＬＤ、Ｚ１１１６：ＯＨ、１６：ＡＬＤ、１６：ＯＨ、Ｚ１１１６ ：ＡＣ的ＥＡＧ反应，发现这些标准化合物均能引起ＥＡＧ 反应，其中Ｚ１１１６：ＡＬＤ能引起最强的ＥＡＧ反应，Ｚ９１６：ＡＬＤ引起的ＥＡＧ反应次之，由此认为Ｚ１１１６ ：ＡＬＤ、Ｚ９１６ ：ＡＬＤ可能是二地区棉铃虫性信息素的主要组分；从雄蛾在不同日龄下对标准化合物Ｚ１１１６：ＡＬＤ、Ｚ９１６：ＡＬＤ 的ＥＡＧ 反应中，发现在羽化后的前３ ｄ 棉铃虫对Ｚ１１１６：ＡＬＤ的ＥＡＧ反应大且反应变动不大，之后则随日龄增加ＥＡＧ反应降低，而对Ｚ９１６ ：ＡＬＤ 的ＥＡＧ的反应则一直保持平稳，ＥＡＧ反应变化的规律与室内观测到的雌蛾性信息素的释放规律相符；测定雄蛾触角在完整、移去触角顶端１／５、２／５ 三种情况下对Ｚ１１１６：ＡＬＤ、Ｚ９１６：ＡＬＤ、Ｚ１１１６ ：ＯＨ 的ＥＡＧ反应，确定了雄蛾触角上感受以上几种组分的感受器主要分布在触角顶端１／５ 处；另外，在Ｚ１１１６ ：ＡＬＤ的绝对含量不变的情况下，研究了Ｚ１１１６ ：ＡＬＤ、Ｚ?

Electroantennograms(EAGs) were recorded from the two areas of male cotton boll worm (Kashi and Kuerle) Helicoverpa armigera in Xinjiang. Z11?16:ALD elicited the greatest EAGs among the standard chemical including Z11?16:ALD,Z9?16:ALD,Z11?16:OH,16:ALD,16:OH and Z11?16:AC, each of which could elicit EAG responsiveness, also we concluded that Z11?16;ALD and Z9?16:ALD were major sex pheromone components of Helicoverpa armigera in Xinjiang; EAGs to Z11?16ALD decreased with age after keeping high and steady responsiveness in its first three days and EAGs to Z9?16:ALD kept steadily, which were consistent with the female calling period that we had disclosed indoors; EAGs to Z11?16:ALD, Z9?16:ALD, Z11?16:OH decreased significantly by successive removal of antennal segments, it was shown that receptors for these compounds were distributed mostly over the distal segment of the five segmented antennae; Z11?16:ALD added with Z11?16:OH proportionally will decrease the effect of Z11?16:ALD alone, while EAGs to Z11?16;ALD added with Z9?16:ALD proportionally did not differ from EAGs to Z11?16:ALD alone. This maybe indicates that the effect of Z11?16:OH was opposite to Z9?16:ALD when both of them were added to Z11?16:ALD.\;