日粮中无机钴添加量对山羊瘤胃液及血清中VB_(12)含量的影响

EFFECT OF THE AMOUNT OF INORGANIC SALT OF COBALT ADDING TO THE RATION OF GOAT ON VB_(12) CONCENTRATION OF RUMEN LIQUID AND SERUM

选３ 只健康白山羊，手术安装瘤胃瘘管后，在日粮中添加不同量的硫酸钴，研究钴的不同添加量对山羊瘤胃微生物ＶＢ１２ 合成量的影响。试验共分４ 期：对照期（ 饲喂基础日粮）；试验１ 期（ 基础日粮＋ 钴０ ．３ ｍｇ／ｋｇ） ；试验２ 期（ 基础日粮＋ 钴０ ．５ ｍｇ／ｋｇ） ；试验３ 期（ 基础日粮＋ 钴０ ．８ ｍｇ／ｋｇ）。每期定时采取瘤胃内容物和血液样品，测定瘤胃液和血清中ＶＢ１２ 的含量。试验结果为：各期瘤胃液中ＶＢ１２ 平均含量（ｎｇ／ｍｌ） 分别为：７ ．４６，１４ ．８５，５２．８６ ，２６ ．１４ ；各期血清中ＶＢ１２ 平均含量（ｎｇ／ｍｌ）分别为：１ ．６６ ，３ ．５９ ，３．９２，４．１８。试验结果表明：在本试验条件下，随着日粮中钴量的增加，血清中ＶＢ１２ 含量随之上升，试验各期与对照期差异极显著（ Ｐ＜ ０．０１） 。证实了日粮中一定范围内的钴添加量与瘤胃液和血清中ＶＢ１２

Three white healthy goats were fitted with rumen fistulas, through adding different amount of CoSO 4 to the goats ration and the effect of the different amount of Co on the VB 12 synthetic amount of rumen microbe was studied. The experiment was divided into four periods: The control period (basic ration), the first trial period (basic ration+0.3 mg/kg Co), the second trial period (basic ration+0.5 mg/kg Co), and the third trial period (basic ration+ 0.8 mg/kg Co). During every period, the sample of the mixture inside rumen and blood of every goat were taken, and then determined the VB 12 concentration. The results were as follows: four periods average VB 12 concentration of the rumen liquid were (ng/ml): 7.46, 14.85, 52.86, 26.14; the concentration of the serum were (ng/ml): 1.66, 3.59, 3.92, 4.18. The results showed that: under the condition of the experiment, the VB 12 concentration of the serum increased significantly (p<0.01) as the amount of Co in the ration rose. So the experiment verified that the relationship between the amount of Co within a certain range adding to the ration and the VB 12 concentration of the rumen liquid and serum of goats was significant.