白藜芦醇对大鼠脑缺血再灌注氧化应激损伤的影响

Effect of Resveratrol on Focal Cerebral Ischemia Reperfusion Injury Via Ameliorating Oxidative Stress in Rats

目的探讨白藜芦醇(Resveratrol,Res)对大鼠脑缺血再灌注氧化应激损伤的影响。方法将SD大鼠随机分为假手术组(S组)、缺血再灌注组(I/R组,线栓法复制大鼠右侧大脑中动脉栓塞模型)、Res低剂量组(15 mg/kg,I/R+R1组)和Res高剂量组(30 mg/kg,I/R+R2组),于缺血2 h再灌注24 h进行神经功能缺损评分;化学比色法测定大鼠血清和脑组织中丙二醛(Malondialdehyde,MDA)含量及超氧化物歧化酶(Superoxide dismutase,SOD)活性;TTC法测定脑梗死体积;干湿重法测定脑含水量,HE染色观察脑组织的病理改变。结果与I/R组相比,Res能改善大鼠脑缺血再灌注损伤后的神经功能缺失(P<0.01),降低血清及脑组织中MDA的含量(P<0.01),提高SOD活性(P<0.01),缩小脑梗死体积(P<0.01),降低损伤侧脑含水量(P<0.01),改善脑组织的病理变化,且呈剂量依赖性。结论 Res对大鼠局灶性脑缺血再灌注氧化应激损伤具有良好的保护作用,其机制可能与清除自由基,减轻氧化性损伤有关。

Objective To investigate the effect of resveratrol（Res） on focal cerebral ischemia reperfusion injury via ameliorating oxidative stress in rats.Methods SD rats were divided into sham operation（S）,ischemia / reperfusion [I / R,i.e.rat model of cerebral artery occlusion（MCAO） copied by intraluminal thread] as well as Res at low（I / R ＋ R1,15 mg / kg） and high（I / R ＋ R2,30 mg / kg） dosage groups,of which the lesions of neurological function were scored 2 h after ischemia and 24 h after reperfusion separately,while determined for malondialdehyde（MDA） content and superoxide dismutase（SDO） activity in serum and brain tissue by chemical colorimetry,for infarct volume by TTC method,for moisture content in brain tissue by dry and wet weight method,and for pathological change of brain tissue by HE staining.Results Res improved the neurological deficit caused by cerebral ischemia reperfusion injury（P 0.01）,decreased the MDA content（P 0.01） and increased the SDS activity in serum and brain tissue（P 0.01）,while decreased the infarct volume（P 0.01） and the moisture content in brain tissue（P 0.01）,and improved the pathological changes of brain tissue,each in a dose-dependent pattern.Conclusion Res showed good protective effect on the cerebral ischemia reperfusion injury via ameliorating oxidative stress by a potential mechanism which might be associated with the clearance of free radicals and the relief of oxidative damage.