Shh修饰骨髓间充质干细胞与不同骨移植材料复合的体外观察

Observation on different bone graft substitutes compound with Shh gene modified MSCs in vitro

目的:研究两种不同骨移植材料对Shh基因修饰的骨髓间充质干细胞(MSCs)生长和增殖活性的影响。方法:差异贴壁法培养大鼠MSCs,携带音狸因子(Shh)腺相关病毒2型(rAAV2-Shh)转导MSCs,然后与两种不同的骨移植材料—聚D,L-乳酸/羟基磷灰石(PDLLA/HA)和β-磷酸三钙(β-TCP)体外复合。MTT法检测MSCs的增殖活性和黏附能力。扫描电镜观察MSCs在骨移植材料上的生长情况。结果:β-TCP组黏附和增殖活性均强于PDLLA/HA组(P<0.05)。此结果在扫描电镜观察中得到同样的证实。结论:与PDLLA/HA比较,β-TCP具有更良好的生物相容性。

Objective：The purpose of this study was to investigate the effect of two kinds of different bone graft substitutes on the growth and proliferation activity of Shh gene modified bone mesenchymal stem cells（MSCs）.Method：The MSCs were cultured and transduced with rAAV2-Shh after differential adherence isolation.Then the cells were seeded with two different bone graft substitutes-poly-DL-lactide/hydroxyapatite（PDLLA/HA） and β-tricalcium phosphate （β-TCP）.The cell proliferation and viability was evaluated by MTT detection.The cultures were examined for cell growth pattern by the scanning electron microscope.Result：β-TCP showed the higher proliferation and adhesion rate than PDLLA/HA （P0.05）,which also confirmed by the scanning electron microscope.Conclusion：Compared with PDLLA/HA,β-TCP has better biocompatibility.