林州市地区食管癌活检组织中人乳头瘤病毒的研究

Study of human papillomavirus in biopsy tissue specimens of esophageal carcinomas in Linzhou city

目的探讨在我国河南省林州市地区食管癌（esophageal carcinoma，EC）活检标本中人乳头瘤病毒（humanpapillomavirus，HPV），特别是高危型HPV的感染状况。方法收集的食管癌活检标本，使用通用引物的套式PCR反应检测HPV的核酸，分别使用型特异性PCR检测HPV16和18的感染。结果18例活检标本全部为HPV阳性，其中HPV16的阳性率为13／18，HPV18的阳性率为4／18，HPV16／18的复合感染为4／18。结论我国河南省林州市地区食管癌活检组织中有HPV存在，其中HPV16的感染占很大比例，并且HPV感染可能是食管癌发生的重要病因。

Objective To investigate the prevalence of human papillomavirus （HPV）, particularly of high-risk HPV in biopsy tissue specimens of esophageal carcinomas in Linzhou city. Methods General nested primer sets were used to detected the whole HPV genotypes, following by HPV16 and 18 type specific PCR for the HPV16 and 18 detection respectively. Results All 18 biopsy samples were HPV positive, and HPV 16 was detected in 13 of the 18 samples, HPV 18 was detected in 4 of the 18 samples. Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV infection may be an important etiologic factor in the development of esophageal cancer in Linzhou city.