来自不同地区的两组鼻咽癌活检组织样本共同失调的基因功能及通路

Common dysregulated genes and pathways in two sets of nasopharyngeal carcinoma biopsy samples from differential regions

目的探究两组鼻咽癌活检组织样本中差异基因表达谱所显示的失调基因功能及通路的相同之处。方法利用生物信息学的方法分析两组差异表达基因谱,对比其失调的基因功能及通路,同时探索其相同的失调功能与两组数据间相同的基因的关系。结果两样本中显著上调的基因主要与细胞的周期调控、增殖、DNA损伤修复、细胞的粘附迁移、代谢及蛋白质结合等相关,但是显著下调的基因无明显的功能聚类;两差异基因表达谱显示出的相同失调基因功能并不是完全由它们的相同基因引起;两者共同失调的通路有10条,排在前4位的为白细胞内皮迁移、细胞粘附分子、粘着斑和磷脂酰肌醇信号系统。结论来自两个不同地区的鼻咽癌活检组织样本的比较发现,其差异表达的基因多与细胞周期调控、DNA损伤修复、粘附迁移等相关,这与临床治疗首选化疗的原因相符;通路分析得出4条差异最显著的共同通路也与肿瘤粘附迁移相关,通过干扰其在肿瘤中的病理作用,特别是磷脂酰肌醇信号系统在肿瘤发生、粘附及转移等关键步骤中的信号传导作用,有望提高鼻咽癌的治疗效果及预后。

Objective To explore the common dysregulated genes and pathways shared by two sets of nasopharyngeal carcinoma（NPC） biopsy samples collected from different regions.Methods Using bioinformatics analysis,the dysregulated genes and pathways in the two sets of samples were compared,and the relationship between the common dysregulated functions and genes was explored.Results The common up-regulated genes in the two sets of samples were involved with such cell functions as cell cycle regulation,cell proliferation,DNA damage and repair,cell adhesion and migration,cell metabolism,and protein binding,but their common down-regulated genes did not show functional clustering.Those common dysregulated gene functions shown by differential gene expression profiling were not completely dictated by identical genes.The top 4 of the 10 common dysregulated pathways included leukocyte transendothelial migration,cell adhesion molecules,adherens junction,and phosphatidylinositol signaling system.Conclusions The differentially expressed genes in NPC are mainly related to cell cycle regulation,DNA damage and repair,cell adhesion and migration,a finding supporting the primary choice of chemotherapy in clinical treatment.The 4 most distinct common dysregulated pathways in the NPC samples are associated with tumor adhesion and migration,and by interventions of these pathways,especially the phosphatidylinositol signaling system in tumorigenesis,adhesion and migration,improvements in the therapeutic effect and prognosis of NPC can be expected.