鼻咽癌细胞株5-8F-EGFP和肝转移亚群5-8F-H3B-EGFP的基因表达谱分析

Gene expression profiling of nasopharyngeal carcinoma cell line 5-8F-EGFP and the liver metastatic 5-8F-H3B-EGFP cells

目的区分不同转移能力的鼻咽癌细胞亚群,对于探讨鼻咽癌转移的分子机制、脏器亲和性具有重要的临床和生物学意义。我们拟应用基因芯片技术来分析鼻咽癌细胞株5-8F-EGFP和肝转移亚群5-8F-H3B-EGFP的基因表达谱差异。方法分别提取鼻咽癌细胞株5-8F-EGFP和肝转移亚群5-8F-H3B-EGFP的总RNA,逆转录成荧光标记的cDNA,与Af-fymetrix Human Genome U_133A 2.0寡核苷酸芯片杂交(每株重复3次),用专业的软件和聚类分析法分析基因表达谱。结果得到差异表达基因3767个,其中差异表达2倍以上的基因281个,进一步筛选出16个可能与鼻咽癌肝转移关系密切的基因16个。结论这16个基因能够很好的区分5-8F-EGFP和肝转移亚群5-8F-H3B-EGFP,为进一步寻找鼻咽癌肝转移的临床分子标志物、判断预后缩小了范围。

Objective To compare the gene expression profiles of nasopharyngeal carcinoma（NPC） cell line 5-8F-EGFP and the liver metastatic 5-8F-H3B-EGFP cells.Methods The fluorescence-labeled cDNA were prepared separately from the total RNA extracted from the two cell lines and hybridized with Human_U133A2.0Genechip（Affymetrix,USA） containing approximately 18 400 known gene.The gene expression profiles were analyzed with special software and cluster analysis.Results A total of 3767 genes were identified to have significant differential expressions between these two cell lines（P0.05）,among which 281 genes showed twofold or higher differential expressions.Using MILANO software,we found 16 genes with probable close relation with liver metastasis of NPC.Conclusion The 16 genes differentially expressed between the two cell lines can be of importance in the investigation of the molecular mechanism of NPC liver metastasis and identification of molecular markers for prognostic evaluation.