华北落叶松和日本落叶松种子超干保存活力和生理特性研究

Vigor and physiological properties of ultra-drying seeds of Larix principis-rupprechtii Mayr.and L.kaempferi(Lamb.) Carr.

采用室温硅胶干燥法,研究了超干处理和加速老化(50℃,30d)对华北落叶松和日本落叶松种子活力和生理特性的影响。对2种落叶松不同含水量种子老化前后的发芽率、发芽势、发芽指数、活力指数、相对电导率、脱氢酶活性和异柠檬酸裂解酶进行测定。结果表明:含水量50～28g/kg的华北落叶松和含水量48～28g/kg的日本落叶松种子老化前后均能维持较高的发芽势、发芽率、发芽指数、活力指数和酶活性,说明2种落叶松种子的超干保存是可行的。老化前后,含水量28g/kg的2种落叶松种子各项指标均最好,表现出很强的抗老化能力,说明该含水量可以作为华北落叶松和日本落叶松种子超干贮藏的可选含水量。

Using ultra-drying over silica gel （at room temperature） and accelerated aging （50℃,30 d） methods,we studied the storage behavior of Larix principis-rupprechtii Mayr.and L.kaempferi（Lamb.） Carr.seeds.Seed germination percentage （GP）,germination energy （GE）,germination index （GI）,vigor index（VI）,relative conductivity（RC）,dehydrogenase activity（DA） and isocitrate lyase activity （ICL） after ultra-drying（pre-aging） and accelerated aging were investigated.The results showed that higher GE,GP,GI,VI,DA and ICL were found in the seeds with moisture content（MC） of 50 28 g/kg for L.principis-rupprechtii and 48 28 g/kg for L.kaempferi before and after aging,indicating the feasibility of ultra-drying storage for seeds of these two species.Furthermore,all indices of seeds with MC of 28 g/kg were best for both species,indicating their better anti-aging ability than seeds with other MCs.The results indicate that 28 g/kg of MC was optimum for the ultra-dying storage of L.principis-rupprechtii and L.kaempfer seeds.