糖尿病大鼠胃窦胆碱能神经元与胃动力障碍的关系

Research on gastric antrum cholinergic neurons in diabetic rats

目的探讨链脲佐菌素(STZ)-糖尿病大鼠胃动力障碍和胃肌间神经丛胆碱能之间的关系。方法 45只SD大鼠随机分为对照组、糖尿病组和胰岛素组。成模后16 w测定大鼠胃动力,观察胃肌间神经丛胆碱能神经元的形态变化。结果与对照组比较,糖尿病组大鼠胃动力减弱(P<0.01),胃窦肌间神经丛胆碱能神经元计数显著降低(P<0.01)。与糖尿病组相比较,胰岛素组胃动力显著增高(P<0.05),胃窦肌间神经丛胆碱能神经元平均光密度显著增高(P<0.05),胆碱能神经元计数有改善的趋势(P>0.05)。结论 STZ-糖尿病大鼠胃动力障碍可能与胃肌间神经丛胆碱能神经损伤有关,胰岛素治疗能在一定程度上改善糖尿病胃动力障碍。

Objective To investigate the relationship between gastric dyskinesis and histology changes of gastric myenteric plexus cholinergic neurons in STZ-induced diabetic rats. Methods 45 SD rats were randomly divided into control, diabetic and insulin groups. 16 weeks after diabetic model established, gastric motility of rats was measured and histology changes of myenteric plexus cholinergic neuron was observed. Results Compared with control group, gastric motility of diabetic group was markedly slow （ P 〈 0.01 ）, the myenteric plexus cholinergic neuron counting of gastric antrum was significantly decreased （ P 〈 0. 01 ）. Compared with diabetic group, both the gastric dynamia and IOD of gastric antrum cholinergic of insulin group were markedly higher （ P 〈 0. 05 ） , yet lower than that of control group （ P 〈 0. 01 ）. Conclusions The gastric dyskinesis of STZ-induced diabetic rats might be associated with lesions of gastric myenteric plexus cholinergic neuron, insulin intensive therapy can partly ameliorate diabetic gastroparesis.