胰岛素样生长因子在肾透明细胞癌患者血液中表达及其临床意义

Expression and its clinical significance of serum insulin-like growth factors in patients with clear cellcarcinoma of kidney

目的探讨胰岛素样生长因子（IGF）Ⅰ、Ⅱ和胰岛素样生长因子结合蛋白（IGFBP）3、5在肾透明细胞癌患者外周血液中的表达及其临床意义。方法选择2007年5月至2009年12月在我院手术治疗的肾透明细胞癌患者40例（肾癌组），对照组为同期的肾积水患者16例，采用酶联免疫吸附法（ELISA）检测两组患者血清标本中IGF—I、Ⅱ和]GFBP3、5的水平。结果肾癌组术前血清IGF—I、Ⅱ和IGFBP3、5分别为985．7μg／L、1154．0μg／L和46．6μg／L、9．6μg／L，术后分别为431．4ug／L、632．6ug／L和26．7ug／L、6．7ug／L，差异均有统计学意义（P值分别为0．001、0．009、0．001、0．002）；肾积水组IGF-Ⅰ、Ⅱ和IGFBP3、5水平术前术后比较，差异无统计学意义（P〉0．05）。结论IGF-I、Ⅱ和IGFBP3、5在肾透明细胞癌患者的血液中呈现高表达，IGF-Ⅱ具有临床诊断意义。

Objective To observe the expressions of serum insulin-like growth factor （IGF）- I , ]I and IGF binding protein （IGFBP） 3, 5 and to explore the clinical significances in patients with clear cell carcinoma of kidney. Methods Enzyme-linked immunosorbent assay （ELISA） methods were adopted to examine serum expressions of IGF-Ⅰ ,Ⅱ and IGFBP 3, 5 in 40 cases with clear cell carcinoma of kidney （renal carcinoma group） and 16 cases with hydronephrosis （control group） from May 2007 to December 2009. Results IGF- [ , lI and IGFBP 3,5 in renal carcinoma showed higher expressions before operation （985.7 μg/L, 1154.0 μg/L,46.6 μg/L and 9.6 μg/L,respectively）than after operation （431.4 μg/L, 632.6 μg/L, 26.7 μg/L, and 6.7 μg/L, respectively, all P〈0.05 0.01）. There were no significant differences in those indexes between pre- and post- operation in control group （P〉0.05）. Conclusions There are high expressions of serum IGF-Ⅰ, Ⅱand IGFBP 3, 5 in renal carcinoma patients, and IGF-H has clinical significance in diagnosis.