SEMA3F蛋白在肝细胞癌中的表达及其预后价值

Expression and prognostic value of SEMA3F protein in hepatocelluar carcinoma

目的研究SEMA3F蛋白在肝细胞癌（hepatocelluarcarcinoma，HCC）中的表达及其预后价值。方法采用WesternBlotting法检测32例HCC及相应癌旁肝组织标本中SEMA3F蛋白的表达。运用免疫组织化学法检测38例HCC以及其相应癌旁组织中的SEMA3F蛋白的表达和微血管密度（microvasseldainty，MVD）值，分析SEMA3F蛋白表达水平与MVD的相关性。结果SEMA3F蛋白在32例HcC组织中的表达明显低于相应的PCLT（447．78±48．26比618．93±61．23，P〈0．05）。SEMA3F在HCC组织中的表达水平与肿瘤的结节数目及是否具有包膜结构密切相关（P〈0．05）。SEMA3F低表达组的复发转移率显著高于高表达组（P〈0．01）而其术后生存率则显著低于高表达组（P〈0．01）。SEMA3F高表达组的MVD值明显低于低表达组（86．56±17．94比115．6±30．38，P〈0．01）。结论sEMA3F在HCC组织中表达下调与肝癌复发转移率高和预后差密切相关，提示其可能是一个潜在的HCC预后分子标志物。

Objective To investigate the expression of Semaphorin 3F （SEMA3F） protein in hepatocellular carcinoma （HCC） and to demonstrate its relationship with clinicopathological features and prognosis of HCC, Methods Western Blotting was carried out in 32 hepatocellular carcinoma samples and matched perineoplastic tissues to detect the expression of SEMA3F protein. The relationship between SEMA3F protein expression and clinicopathological features as well as prognosis of HCC patients was analyzed. Immunohistochemistry was used to show the location of SEMA3F in HCC cells and its relationship with microvessel density （MVD）. Results The expression of SEMA3F protein in HCC tissues was significantly higher than in the perineoplastic tissues （447.78 ± 48.26 vs 618.93 ± 61.23, P〈0. 05） and it was correlated closely with tumor capsulation and tumor nodular number （P〈0. 05）. Based on the Western Blotting and clinical follow-up data, we found that the survival time of HCC patients with a higher SEMA3F expression level was longer than those with a lower level, and the recurrent/metastatic time of HCC patients was significantly different between these two groups （P〈0. 01）. Immunohistochemistry demonstrated that SEMA3F protein localized in the cytoplasm of HCC cells and its expression correlated with HCC MVD. MVD in the low-level group was higher than the high-level group （115.6±30.38 vs 86.56±17.94, P〈0.01）. Conclusions SEMA3F expression in HCC was significantly down-regulated and correlated closely with tumor-capsulation, nodular number, and MVD, implicating SEMA3F may play an important role in recurrence and metastasis of HCC. It can be regarded as a prognostic marker in HCC patients.