大网膜脂肪组织提取液对成纤维细胞作用的研究

Effect of adipose tissue extract of greater omentum on human fibroblasts in vitro

目的探讨大网膜脂肪组织提取液对成纤维细胞的作用。方法应用倒置显微镜、MTT法、流式细胞仪、透射电镜观测提取液对成纤维细胞生长、增殖、凋亡以及细胞超微结构的影响。结果大网膜脂肪组织提取液对成纤维细胞的生长增殖有抑制作用；抑制细胞凋亡，并影响细胞的有丝分裂，并使细胞功能受到抑制。结论大网膜脂肪组织提取液可以抑制成纤维细胞的生长，有可能影响胆管瘢痕狭窄的形成和发展。

Objective To study effect of the adipose tissue extract of greater omentum on human fibroblasts. Method The effect of the adipose tissue extract of greater omenturn on human fibroblasts was observed by inverted microscope, MTT, flow eytometry, transmission electron microscope. Results The growth of fibroblasts was inhibited by the adipose tissue extract of greater omenturn. The apoptosis of fibroblasts was not inhibited, and the cell function of fibroblasts was inhibited and the mitosis of fibroblasts was affected. Conclusions The growth of fibroblasts can be inhibited by the adipose tissue extract of greater omentum. The formation and development of biliary cicatricial constriction may be affected by the adipose tissue extract of greater omentum.