骨形态发生蛋白9抑制人乳腺癌MDA-MB-231细胞体外侵袭和迁移

BMP9 inhibits invasion and migration of human MDA-MB-231 cells in vitro

目的探讨骨形态发生蛋白9(BMP9)对乳腺癌MDA-MB-231细胞侵袭、迁移能力的影响。方法 RT-PCR法检测MDA-MB-231细胞系中BMP9的表达;扩增高滴度的BMP9表达腺病毒,感染MDA-MB-231细胞,制备高表达BMP9的重组MDA-MB-231/BMP9细胞,以此作为实验组;同时以含GFP的空载腺病毒感染该细胞为MDA-MB-231/GFP,联合空白MDA-MB-231共同作为对照组;通过平板集落形成实验、细胞划痕实验、Transwell侵袭实验检测重组MDA-MB-231/BMP9细胞侵袭及迁移能力。结果与对照组细胞相比,实验组细胞中BMP9 mRNA表达水平明显增高;实验组细胞集落形成率由对照的90.6%±2.5%与85.6%±3.5%显著下降至57.3%±4.5%(P<0.05);实验组划痕愈合率由对照的95.4%±3.1%与92.5%±2.4%下降至74.0%±3.6%(P<0.05);实验组穿膜细胞数由对照的(255.3±16.1)个与(267.3±14.9)个下降至(150.3±14.6)个(P<0.05)。结论 BMP9可以在体外抑制乳腺癌MDA-MB-231细胞的侵袭与迁移。

Objective To investigate the correlation among bone morphogenetic protein 9（BMP9） expression,migration and invasion of breast cancer MDA-MB-231 cells in vitro.Methods The expression of BMP9 in MDA-MB-231 was detected by RT-PCR,high titer of adenovirus vector expressing BMP9 was used to infect MDA-MB-231.Experimental group： MDA-MB-231/BMP9,control group： MDA-MB-231/GFP and MDA-MB-231.To detect the changes of invasion and migration in the recombinant MDA-MB-231/BMP9 cells colony-forming assay,cell wounding assay,Transwell invasion assay were carried out.Results Compared with MDA-MB-231/GFP cells and MDA-MB-231 cells,the mRNA level of BMP9 was increased in MDA-MB-231/BMP9 cells.The cell colony formation rate of experimental group was decreased.The healing rate of experimental group was lower than that of control group.The number of invading cells which moved across the matrix barrier for experimental group was less that than that of control group.Conclusion BMP9 can inhibit the invasion and migration of breast cancer in vitro.