非小细胞肺癌组织mTOR/P70S6K表达临床意义的探讨

Expression and clinical significance of mTOR/P70S6K in non-small cell lung cancer tissues

目的:分析在非小细胞肺癌(NSCLC)组织中mTOR/P70S6K/RPS6/eIF4G信号转导途径相关基因mTOR、P70S6K、RPS6、eIF4G的表达水平,探讨mTOR/P70S6K/RPS6/eIF4G基因在NSCLC组织中的表达及临床意义。方法:通过外科手术中获取65例NSCLC组织及30例癌旁组织标本,采用逆转录聚合酶链反应(RT-PCR)技术检测肺癌组织及癌旁组织中mTOR、P70S6K、RPS6和eIF4G基因的表达水平。结果:mTOR、P70S6K、RPS6和eIF4G基因在NSCLC组织中的表达水平分别为(0.54±0.36)%、(0.75±0.34)%、(0.82±0.43)%和(0.28±0.59)%,在癌旁组织中的表达水平分别为(0.28±0.21)%、(0.03±0.03)%(、0.07±0.07)%和(0.09±0.17)%,肺癌组织中的表达水平均显著高于癌旁组织。mTOR、P70S6K、RPS6和eIF4G基因的表达水平同NSCLC患者的年龄、性别、病理类型、分化程度及TNM分期没有明显关系。结论:NSCLC中mTOR/P70S6K/RPS6/eIF4G信号转导途径相关基因显著上调,说明该通路在NSCLC中被激活,因而mTOR/P70S6K/RPS6/eIF4G信号转导途径异常活化可能与NSCLC发病机制有关,可作为诊断NSCLC诊断的标志,且联合检测的结果更可靠。

OBJECTIVE： To detect the mRNA expression levels of roTOR, P70S6K, RPS6 and eIF4G genes, the key genes of mTOR/PTOS6K/RPS6/eIF4G signaling pathway, in human NSCLC tissue, and explore the relationship between roTOR/ P70S6K/ RPS6/eIF4G signaling pathway and NSCLC. MESULTS： Lung cancer tissue specimens were obtained from 65 patients. Adjacent-tumor NSCLC tissues from the 30 patients were served as control. The RT-PCR technique was used to de- tect the roTOR, PTOS6K, RPS6 and eIF4G genes expression levels. RESULTS： The average mRNA expression levels of roTOR, P70S6K, RPS6 and eIF4G genes in NSCLC tissues were （0.54±0.36）%, （0.75±0.34）%, （0.82±0.43）% and （0.28±0.59）%, respectively, while the mRNA expression levels of roTOR, P70S6K, RPS6 and eIF4G genes in adjacenttumor tissues were（0.28±0.21）%, （0.03±0.03）%, （0.07± 0.07）% and （0.09 ± 0. 17）%, respectively. All the levels of roTOR, PTOS6K, RPS6, eIF4G genes expressions in NSCLC tissues were significantly higher than that in adjacent-tumor lung tissue. There were not significantly relationship between roTOR, PTOS6K, RPS6, eIF4G genes expression levels and patientsage, gende, pathological type, differentiation and pTNM stage. CONCLUSIONS： It has demonstrated that mTOR/ P70S6K/ RPS6/eIF4G signaling pathway is activated in the tumor cells of NSCLC. The activated roTOR/ PTOS6K/RPS6/eIF4G signaling pathway may be an important role in the pathogenesis of NSCLC.