细支气管肺泡癌组织凋亡相关基因表达水平与临床病理因素相关性分析

Relationships between the expression of apoptosis-related genes and clinical pathology factors of bronchioloalveolar carcinoma

目的:研究影响细支气管肺泡癌(BAC)组织中凋亡相关基因Bcl-2、bax和Caspase-3表达的相关因素。方法:收集经病理学确诊且临床资料完整的BAC患者38例,RT-PCR法测定其肿瘤组织中Bcl-2、bax和Caspase-3 mRNA水平,并分析其与BAC患者性别、吸烟史、肿瘤临床分型及TNM分期之间的关系。结果:女性BAC组织的Bcl-2mRNA水平显著高于男性,P<0.001,而Bax和Caspase-3 mRNA水平男、女性间差异无统计学意义,P>0.05;吸烟者BAC组织的Bcl-2 mRNA水平显著低于不吸烟者,P<0.001,而Bax和Caspase-3 mRNA水平显著高于不吸烟者,P均<0.001;孤立结节型、多发结节型、肺炎型BAC组织的Bcl-2 mRNA水平依次显著增高,P<0.001,而Bax和Caspase-3 mRNA水平依次显著下降,P均<0.001;随着TNM分期由Ⅰ期至Ⅳ期的逐级升高,BAC组织的Bcl-2 mRNA水平依次显著增高,P<0.001,而Bax和Caspase-3 mR-NA水平依次显著下降,P均<0.001。结论:BAC组织中凋亡相关基因Bcl-2、Bax、Caspase-3 mRNA水平与肿瘤患者的性别、吸烟史、临床分型、TNM分期均有不同程度和方向的相关性。

OBJECTIVE： To study the factors related with the expression of apoptosis-related genes（gcl-2, bax, Caspase-3） in bronchioloalveolar carcinoma（BAC）. METHODS： Totally 38 pathologically diagnosbd BAC cases were collected. The levels of Bcl-2, bax and Caspase-3 mRNA in the carcinomas were deter- mined, and the relationships of the levels with the gender, smok- ing, clinical type and TNM stage were analyzed statistically. RE- SULTS： The Bcl-2 mRNA of female group was significantly high- er than that of male group （P〈0. 001）, but there were no signif- icant differences between the two groups in Bax and Caspase-3 mRNA levels （P〈0.05）. The Bcl-2 mRNA of smoking group was significantly lower than that of unsmoking group （P 0. 001）, but the Bax and Caspase-3 mRNA levels were significantly higher than those of unsmoking group （P〈0. 001）. The Bcl-2 mRNA levels of solitary nodular type, multiple nodular type and pneumonia type were significantly increased by turns （P〈0. 001）, but the levels of Bax and Caspase-3 mRNA were significantly decreased by turns （P%0. 001）. With the stage of the carcinoma advancing from Ⅰ to Ⅳ, the Bcl-2 mRNA levels significantly increased by turns （P〈0. 001）, hut the the levels of Bax and Caspase-3 mRNA were significantly decreased by turns （P〈0. 001）. CONCLUSION： There exist correlations of different grades between the expression of apoptosis-related genes （Bcl-2, Bax, Caspase-3） mRNA and the gender, smoking, clinical type and TNM stage in bronchioloalveolar carcinoma.