RKIP在肺鳞癌组织中的表达及其意义

The Expression and Significance of RKIP in Lung Squamous Cell Carcinoma Tissues

背景与目的 Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)属于磷脂酰乙醇胺结合蛋白家族的成员,可干扰Raf-1-MEK1/2-ERK1/2信号通路,抑制NF-κB和G蛋白偶联受体激酶等信号传导过程,且RKIP的表达减弱或丢失与多种肿瘤的发生发展以及侵润转移相关。本研究旨在探讨RKIP在肺鳞癌组织中的表达及其与肺鳞癌临床病理的相关性。方法应用RT-PCR方法检测56例肺鳞癌组织及其癌旁正常组织中RKIP基因的表达,计算其阳性率;并用Western blot方法检测肺鳞癌组织及其癌旁正常组织中RKIP蛋白的表达变化。结果 RKIP mRNA在肺鳞癌组织中表达的阳性率明显低于癌旁正常组织(P<0.05),在有淋巴结转移者中的表达阳性率明显低于无淋巴结转移者(P<0.05),但与患者的性别、年龄、肿瘤的大小及分化程度无明显关系(P>0.05)。RKIP蛋白在肺鳞癌组织中表达明显低于癌旁正常组织(P<0.05)。结论 RKIP的低表达可能与肺鳞癌的发生及侵袭转移有关。

Background and objective Raf kinase inhibitory protein（RKIP） belongs to the phosphatidylethanolamine binding protein（PEBP） family.RKIP is an endogenous inhibitor of the Raf-1-MEK1/2-ERK1/2 signaling pathway,NFkappaB signaling pathway and G protein coupled receptors signaling pathway.The aim of this study is to explore the expression of RKIP in lung squamous cell carcinoma,and the relationship between RKIP expression and clinical pathology of lung squamous cell carcinoma.Methods The expression of RKIP mRNA was detected in 56 lung squamous cell carcinoma tissues and adjacent cancer tissues by RT-PCR.The expression level of RKIP protein was detected by Western blot.The positive rates of RKIP expression in cancer tissues and adjacent cancer tissues were analyzed.Results RKIP mRNA positive expression rate in cancer tissues was significantly lower than that in adjacent cancer tissues（P0.05）.The positive rate of RKIP mRNA expression in the cancer tissues with lymph node metastasis was significantly lower than that without lymph node metastasis（P0.05）.RKIP mRNA expression has no remarkable correlation with age,sex,tumor differentiation and size（P0.05）.RKIP protein expression in the cancer tissues was significantly lower than that in adjacent cancer tissues（P0.05）.Conclusion The deficiency of RKIP expression is positively correlated with carcinogenesis and invasion metastasis of lung squamous cell carcinoma.