胆道疾病患者343份胆汁细菌培养与药敏分析

Bacterial Culture and Drug Sensitivity Analyses of 343 Samples from Patients with Biliary Tract Diseases

了解胆汁中细菌分布及药敏情况,以指导临床合理用药。对343例胆道疾病手术患者抽取胆汁进行常规培养及应用英国OXID血液增菌瓶和ATB自动细菌鉴定统对标本进行细菌鉴定和药敏分析。343份胆汁中共培养出10种113株细菌(不包括厌养菌)阳性率为37.4%,主要菌种有大肠埃希菌、肺炎克雷伯菌、粪肠球菌、阴沟肠杆菌、铜绿假单胞菌比率较高。药敏结果显示,革兰阴性杆菌对亚胺培南、头孢三代、阿米卡星、庆大霉素敏感性较好;革兰阳性球菌对万古霉素、亚胺培南、利福平敏感性较好。胆道感染多由肠道细菌逆行感染引起的,为此,根据药敏试验结果指导临床用药是非常重要的。

United Kingdom made OXID blood culture enrichment bottles and ATB automatic bacterial identification system were used to analyze and identify bacteria in the samples collected from 343 cases＇ surgery patients with bile duct disease and routinely cultured in order to understand the distribution and drug resistance of bacteria in bile,so as to guide rational drug use.The results showed that altogether from 343 bile samples 10 genera with 113 bacterial strains（not including anaerobic bacteria） were cultured with positive rate at 37.4%,mainly were E.coli,Klebsiella pneumoniae,Enterococcus faecalis,Enterobacter cloacae,Pseudomonas aeruginosa and single-cell bacteria were among the higher rates.Drug susceptibility results showed that sensitivity of Gram-negative rods to imipenem,third generation cephalosporins,amikacin,and gentamycin was fairly good;and sensitivity of Gram-positive cocci to vancomycin,imipenem,and rifampin sensitivity was fairly good.Therefore biliary tract infections caused by retrograde infection from the intestinal bacteria,and for this reason,according to the results of clinical drug susceptibility testing is very important.