依达拉奉对大鼠外伤性癫癎海马CA3区的神经保护作用

Effects of Edaravone on patho-reconstitution of the CA3 areas of hippocampus of posttraumatic epilepsy in rats

目的观察外伤性癫癎(PTE)大鼠模型海马CA3区CD133、核因子-kB(NF-kB)及胶质纤维酸性蛋白(GFAP)的动态表达及自由基清除剂依达拉奉对其的影响,探讨PTE可能的防治机制。方法健康成年雄性SD大鼠采用随机数字表法分为正常组(A,n=6)、对照组(B,n=15)和治疗组(C,n=15)。观察行为学、脑电图,在不同时间点取脑,分别行抗CD133、NF-kB及GFAP酶标免疫组织化学染色,对海马CA3区行病理学观察。结果与A组比较,B、C组1 d后海马CA3区CD133表达逐渐增加,7 d时表达最强,差异均有统计学意义(P<0.05),14 d时降低;NF-kB在1 d时大量表达,7 d后减少,14 d明显减少;GFAP在7 d表达增强,14 d时最多,差异均有统计学意义(P<0.05)。C组三种指标同一时期的表达均较A、B组弱,差异具有统计学意义(P<0.05)。结论 PTE后的氧化应激反应可诱导海马神经前体细胞的增殖分化,或者神经胶质细胞自身增殖,造成海马结构紊乱。依达拉奉可能抑制这一病理重构过程,从而在晚期PTE的防治中起重要作用。

Objective To observe the kinesis expression of CD133,nuclear factor-kB （NF-kB） and glial fibrillary acidic protein （GFAP） in the CA3 area of hippoeampus of the experimental posttraumatic epilepsy （FILE） in rats and the effect of the free radical scavenging agent edaravone, to discuss possible prevention and cure mechanisms of PTE. Methods Fifty-one healthy adult male Sprague-Dawley rats were randomly divided into normal group （A, n = 6）,control group （B, n = 15 ） and treatment group （ C, n = 15）. Behavior was observed and EEG were recorded in each group, brains were removed at different time points. The expression of CD133, NF-kB and GFAP in the CA3 area of hippocampus were detected immunohistochemically to observe the pathological changes. Results Compared with group A, the expression of CD133 in the CA3 area of hippocampus of group B and C gradually increased at one day after injection and increased significantly 7 days later （P 〈 0. 05）, and decreased after 14 days. The expression of NF-kB sharply increased one day after injection,decreased in 7 days later and significantly decreased after 14 days. The expression of GFAP increased obviously after 7 days and this high expression level was maintained till 14 days compared to group A （ P 〈 0. 05 ）. Compared to group A and B ,the expression of CD133 ,NF-kB ,GFAP in group C were weaker at each time piont. Conclusions Oxidative stress reaction after FIE may induce the proliferation and differentiation of neural precursor cells and the self-proliferation of glial cells,which leading structure disorder;Edaravone can effectively alleviate the patho-reconstitution process ;playing an important role in the prevention and cure in late FTE.