r-HuEPO对外伤性脑损伤大鼠凋亡基因表达的影响

Effect of r-HuEPO on apoptotic gene expression of rats after traumatic brain injury

目的探讨重组人促红细胞生成素(r-HuEPO)对外伤性脑损伤大鼠凋亡基因表达的影响。方法将48只健康成年雄性SD大鼠随机分成4组:r-HuEPO1 000 U/kg、3 000 U/kg、5 000 U/kg治疗组和生理盐水对照组。采用改良的Feeney氏法制作大鼠自由落体脑创伤模型,r-HuEPO干预。一周后麻醉取脑冻存。免疫组织化学法测定脑组织中NF-κB、c-myc和Fas/Fasl的阳性细胞及凋亡细胞数。结果与对照组相比,各组中NF-κB、Fas和Fasl阳性细胞及凋亡细胞数均有显著减少(P<0.05),尤其是5 000 U/kg r-HuEPO组减少最显著(P<0.01)。结论 r-HuEPO可抑制创伤性脑损伤大鼠NF-κB、Fas/Fasl的促凋亡作用,减轻迟发性神经元损伤,从而起到神经保护的作用。

Objective To explore the effect of recombinant human erythropoietin （r-HuEP0） on apoptosis gene expression of rats after traumatic brain injury. Methods 40 healthy adult male Sprague-Dawley rats were divided randomly into 4 groups, i. e. 1000 U/kg （A） , 3000 U/kg （B）, 5000 U/kg r-HuEPO-treated（C） and normal saline （D） groups. Modified Feeney＇ s traumatic brain injury model was used. The brains of rats were taken out and frozen under general anesthesia after one week. Positive cells of NF-κB, c-myc, Fas/Fasl-and apoptosis were detected with method of immunohistochemistry. Results Compared with the D group,the positive cell number of NF-κB, cmyc, Fas/Fas] and apoptosis of the A, B and C groups significantly reduced （ P 〈 0.05） , especially in C group （P 〈 0.01 ）. Conclusion r-HuEPO might reduce the delayed neuronal damage and educe its neuroprotective effect by extenuating the bad role of NF-KB and Fas/Fasl after traumatic brain injury.